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| Product Name | Chicken GH (Growth Hormone) ELISA Kit |
| Catalog No. | HTK-YJL-0003 |
| Synonyms | Somatotropin ELISA Kit, Growth Hormone ELISA Kit, GH ELISA Kit |
| Species | Chicken |
| UniProt ID | P08998 |
| Sample Type | Serum, plasma, cell culture supernatant, cell or tissue lysate, other liquid samples |
| Detection Method | Sandwich ELISA, Double Antibody |
| Detection Wavelength | OD450 |
| Reaction Duration | 4 hours |
| Range | 0.156-10 ng/ml |
| Sensitivity | 0.094 ng/ml |
| Storage | 2-8°C (Sealed). Don't cryopreserve. |
| Specificity | Specifically binds with GH, no obvious cross reaction with other analogues. |
| Intended Use | For research use only. |
Growth hormone (GH), also scientifically known as somatotropin, is a key protein hormone predominantly secreted by the somatotroph cells in the anterior pituitary gland of chickens. Composed of approximately 191 amino acids, it plays an irreplaceable role in regulating the postnatal growth and metabolic processes of chickens, making it a core research target in poultry endocrinology, developmental biology, and animal nutrition.
In modern poultry research and breeding practices, understanding the secretion pattern and concentration changes of chicken GH is of great significance for multiple fields:
Poultry Growth Performance Regulation: Chicken GH directly promotes the synthesis of proteins in tissues and organs (such as muscles, bones, and internal organs), accelerates fat degradation, and regulates carbohydrate metabolism—all of which are closely related to the growth rate, body weight gain, and feed conversion efficiency of broilers and laying hens.
Breeding Selection: By detecting the GH level in chicken populations, researchers can screen breeding individuals with excellent genetic potential for growth and development, providing a scientific basis for the cultivation of high-performance poultry breeds.
Nutrition and Environmental Impact Assessment: Factors such as dietary nutrient composition (e.g., levels of zinc, copper, phytase), light conditions (e.g., monochromatic light wavelength), and environmental stress (e.g., temperature, density) can affect the secretion of chicken GH. The detection of GH concentration helps evaluate the effectiveness of nutritional formulas and the impact of environmental factors on poultry physiology.
Pathophysiological Research: Abnormal changes in chicken GH levels may be associated with growth disorders, metabolic diseases, or pituitary function abnormalities. Accurate detection of GH is crucial for studying the pathogenesis of related diseases and developing preventive measures.
Traditional methods for detecting chicken GH, such as radioimmunoassay (RIA), have limitations including radioactive contamination risks, short reagent validity periods, and complex operational procedures. In contrast, the Sandwich ELISA (Enzyme-Linked Immunosorbent Assay) technology adopted by this Chicken GH ELISA Kit has become the preferred method in modern laboratories due to its high safety, strong specificity, and simple operation. It can quickly and accurately quantify GH in various chicken-derived samples, meeting the research needs of universities, research institutions, and poultry breeding enterprises.
Broad Sample Compatibility: This kit is applicable to multiple types of chicken-derived samples, including serum, plasma, cell culture supernatant, and cell or tissue lysate. It does not require complex pre-treatment of samples (except for standard lysate preparation), which greatly reduces the difficulty of sample handling and expands the scope of research applications.
High Detection Sensitivity: With a minimum detectable concentration (sensitivity) of 0.094 ng/ml, the kit can accurately capture low-level GH in samples (such as GH in early chick plasma or cell culture supernatant with low secretion). This avoids the problem of "false negative" results caused by insufficient sensitivity of conventional detection methods.
Wide Linear Detection Range: The kit covers a detection range of 0.156 - 10 ng/ml, which can match the GH concentration range in most physiological and experimental scenarios of chickens. It eliminates the need for repeated dilution or concentration of samples (even for samples with slightly higher GH levels, simple 1:2 dilution can meet the detection requirements), saving experimental time.
Excellent Specificity: The kit uses a double-antibody Sandwich ELISA design, and the pre-coated antibody and biotin-conjugated detection antibody are both highly specific to chicken GH. There is no obvious cross-reactivity with other chicken cytokines (such as insulin-like growth factor-1, IGF-1) or GH analogues of other species (such as mouse GH, human GH), ensuring the accuracy of detection results.
Stable Reaction System: The total reaction duration of the kit is 4 hours, and the enzyme-substrate reaction (using TMB as the substrate) produces a stable colored product. The color change is not easily affected by slight fluctuations in ambient temperature (within 25 - 37 °C) during the operation process, and the absorbance value (OD450 nm) read within 30 minutes after adding the stop solution has good repeatability.
Convenient Storage Conditions: All reagents of the kit can be stored at 2 - 8 °C (sealed) without cryopreservation. This avoids the loss of antibody activity caused by repeated freezing and thawing of reagents (a common problem with low-temperature stored kits) and simplifies the storage management of laboratories (no need for long-term occupation of -20 °C or -80 °C freezers).
Time-Saving and Efficient Operation: Compared with traditional RIA (which requires 6 - 8 hours of reaction time) and other ELISA kits with long incubation periods (some take 6 - 7 hours), this kit shortens the total reaction time to 4 hours.
Cost-Effective Experimental Scheme: The kit provides two specifications of 48T and 96T, which can be flexibly selected according to the number of samples (e.g., 48T for small-scale preliminary experiments and 96T for large-scale batch detection). At the same time, the reagents in the kit have good stability (6 - month shelf life under proper storage conditions), and there is no waste caused by reagent expiration due to slow sample accumulation.
Reliable Result Reproducibility: The kit has strict quality control standards. The intra-assay coefficient of variation (CV) (precision within the same experiment) is less than 10%, and the inter-assay CV (precision between different experiments) is less than 12%. Even if the same sample is detected in different batches or by different operators, the deviation of the results is small, which is conducive to the comparison and verification of research data.
Strong Compatibility with Common Instruments: The detection wavelength of the kit is 450 nm, which is the standard wavelength of most commercial microplate readers (such as Thermo, Bio-Rad, and other brands of microplate readers). Laboratories do not need to purchase additional special instruments, reducing the threshold for using the kit.
Research-Oriented Design: The kit is designed for scientific research use, and the detection results can provide original data support for various research directions (such as chicken growth regulation, nutritional physiology, and endocrine mechanism). At the same time, the kit provides detailed operation instructions, including sample preparation methods, standard curve drawing steps, and troubleshooting guides, which is friendly to novice researchers.
For research use only, not for clinical use.
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