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| Product Name | Chicken FSH (Follicle-Stimulating Hormone) ELISA Kit |
| Catalog No. | HTK-YJL-0085 |
| Synonyms | FSH ELISA Kit, Follicle-Stimulating Hormone ELISA Kit |
| Species | Chicken |
| UniProt ID | Q8QGF8 |
| Sample Type | Serum, plasma, cell culture supernatant, cell or tissue lysate, other liquid samples |
| Detection Method | Competitive ELISA, Coated with Antigen |
| Detection Wavelength | OD450 |
| Reaction Duration | 2 hours |
| Range | 15.625-1000 pg/ml |
| Sensitivity | 9.375 pg/ml |
| Storage | 2-8°C (Sealed). Don't cryopreserve. |
| Specificity | Specifically binds with FSH, no obvious cross reaction with other analogues. |
| Intended Use | For research use only. |
What is Chicken FSH?
Follicle-Stimulating Hormone (FSH) is a key member of the gonadotropin family, primarily secreted by the anterior pituitary gland of chickens. It plays a critical role in regulating the reproductive physiology of chickens, collaborating closely with Luteinizing Hormone (LH) to drive follicular growth, development, differentiation, and maturation in the ovaries. For research scenarios, understanding chicken FSH levels is essential for studies related to poultry reproductive efficiency, such as exploring the relationship between FSH concentration and laying frequency—research has indicated that high levels of FSH are associated with a high daily laying frequency in Isa Brown strain hens. Additionally, low doses of FSH have been shown to effectively induce follicular turnover, making it a vital biomarker in poultry endocrinology and reproductive science research.
Why Use an ELISA Kit for Chicken FSH Detection?
Enzyme-Linked Immunosorbent Assay (ELISA) has become the preferred method for chicken FSH quantification since its establishment in the early 1970s, due to its unique combination of specificity, sensitivity, simplicity, and speed. Compared to traditional Radioimmunoassay (RIA) technology, ELISA offers distinct advantages: it has a longer reagent half-life, minimizes environmental pollution, and simplifies the disposal of experimental waste. While basic ELISA sensitivity was once lower than RIA, the development of multiple amplification systems (e.g., biotin-avidin enzyme complexes, non-chromogenic substrates like fluorescent or luminescent reagents, and enzyme-catalyzed cascading reaction systems) has now enabled ELISA to match or even exceed RIA in sensitivity, making it more suitable for the precise detection of chicken FSH in various biological samples.
Application Scenarios of Chicken FSH ELISA Kit
This Chicken FSH ELISA Kit is designed exclusively for research use (not for clinical diagnosis) and supports a wide range of research fields, including poultry endocrinology (studying FSH’s role in the hypothalamic-pituitary-ovarian axis), reproductive science (optimizing chicken breeding efficiency and laying performance), genetic science (exploring genetic variations in FSH secretion), and hormone metabolism (analyzing FSH synthesis, degradation, and regulatory mechanisms). It is compatible with multiple sample types commonly used in these research areas, ensuring flexibility for different experimental designs.
Utilizes a competitive ELISA method with antigen pre-coated microplates, which ensures specific binding between the kit’s reagents and chicken FSH, reducing non-specific reactions and enhancing detection accuracy.
Offers a well-defined detection range of 15.625–1000 pg/ml, covering the typical concentration range of chicken FSH in most biological samples (e.g., serum, plasma, cell culture supernatant) and eliminating the need for excessive sample dilution that could introduce errors.
Boasts high sensitivity of 9.375 pg/ml, enabling the detection of low-concentration chicken FSH samples, which is critical for research on early follicular development or subtle hormonal changes in chickens.
Features a short reaction duration of 2 hours, significantly shortening the experimental cycle compared to longer-assay kits (e.g., 3.5-hour kits), allowing researchers to obtain results more efficiently and accelerate research progress.
Supports multiple common biological sample types, including serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples, providing flexibility for different research models and sample collection protocols.
Ensures strong specificity, as the kit’s antibodies specifically bind to chicken FSH with no obvious cross-reactivity with other related hormones or analogs (e.g., LH, other gonadotropins), avoiding interference from co-existing substances in samples.
Has straightforward storage requirements: 2–8°C sealed storage (no cryopreservation needed), simplifying reagent management and reducing the risk of reagent degradation caused by repeated freeze-thaw cycles.
High Detection Accuracy: The combination of competitive ELISA technology and pre-coated antigen plates minimizes background noise and cross-reactivity, ensuring that the measured optical density (OD) values accurately reflect chicken FSH concentrations in samples. This reliability is further supported by consistent performance across repeated experiments, which is essential for producing reproducible research data.
User-Friendly Operation: The kit’s standardized reaction steps and short 2-hour assay time reduce the complexity of experimental operations, making it accessible to both experienced researchers and those new to ELISA techniques. There is no need for specialized or complex equipment beyond a standard microplate reader (set to 450 nm wavelength), lowering the barrier to use.
Broad Sample Compatibility: By supporting serum, plasma, cell culture supernatant, cell/tissue lysate, and other liquid samples, the kit eliminates limitations on sample type selection. Researchers do not need to purchase additional kits for different samples, reducing experimental costs and streamlining workflow.
Stable Reagent Performance: The 2–8°C storage condition (without cryopreservation) maintains reagent stability over time, preventing activity loss from freeze-thaw cycles. This stability ensures that the kit retains its sensitivity and specificity throughout its shelf life, avoiding experimental failures due to degraded reagents.
Research-Specific Optimization: The kit is tailored to the unique needs of chicken FSH research, with its detection range and sensitivity aligned to the physiological concentrations of chicken FSH. This optimization ensures that researchers can obtain meaningful data without compromising on either low-concentration detection or avoiding saturation of high-concentration samples.
For research use only, not for clinical use.
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