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| Product Name | Cellulose Content Assay Kit, 100T/96S |
| Catalog No. | SCAK-YJL-0167 |
| Detection Method | Micro-volume method, Spectrophotometer/Microplate Reader |
| Storage | Store at 2-8°C, 6 months |
| Intended Use | For research use only. |
| Note | For your safety and health, please wear lab coat, disposable gloves and mask during operation. |
Cellulose, as a linear polymer composed of β-D-glucose units linked by β-1,4-glycosidic bonds, is one of the most abundant organic compounds on Earth and serves as the primary structural component of plant cell walls. In natural plant tissues, it often coexists with hemicellulose, pectin, and lignin, forming a complex cell wall matrix that provides mechanical support and protection for plants. With the continuous development of scientific research and industrial applications, the demand for accurate and efficient cellulose content detection has grown significantly across multiple fields.
Research Field Demand: In plant biology, agronomy, and forestry research, cellulose content is a key indicator reflecting plant growth status, stress resistance, and material quality. For example, in crop breeding programs, researchers need to measure cellulose content in straw or stalks to screen varieties with better lodging resistance or higher forage nutritional value. In forestry studies, cellulose content in wood is closely related to timber strength and papermaking performance, making its detection essential for optimizing wood utilization.
Industrial Application Drivers: The cellulose industry covers a wide range of sectors, including food, paper, textiles, plastics, and bioenergy. In the food industry, cellulose is used as a dietary fiber additive, and its accurate quantification ensures compliance with product nutrition labeling standards. In the paper and textile industries, cellulose content directly affects the quality and production efficiency of pulp and fiber materials—manufacturers rely on precise detection results to adjust production processes and control product consistency. In the emerging bioenergy field, cellulose-rich biomass (such as corn stover, sugarcane bagasse) is a critical raw material for bioethanol production; detecting cellulose content helps evaluate raw material quality and optimize bio-conversion efficiency.
Technological Development Context: Traditional cellulose detection methods (e.g., gravimetric method, acid hydrolysis-titration method) often suffer from drawbacks such as long operation time, cumbersome procedures, large sample consumption, and poor repeatability. With the advancement of analytical technology, spectrophotometric/microplate reader-based assay kits have become the mainstream choice for researchers and industrial laboratories. These kits leverage specific chemical reactions (e.g., anthrone chromogenic reaction after acid hydrolysis of cellulose into glucose) to achieve rapid, sensitive, and high-throughput detection, addressing the inefficiencies of traditional methods and meeting the needs of modern laboratory workflows for speed and accuracy.
Market and Policy Support: As global attention to sustainable development and renewable resources increases, policies promoting the development of the biomass industry, green manufacturing, and agricultural product quality testing have been issued in many countries. For instance, support for bioenergy research and development, stricter food safety and quality supervision standards, and investments in agricultural science and technology have all driven the demand for reliable cellulose detection tools. This kit, with its 100T/96S capacity and micro-volume detection design, is well-suited to meet the high-throughput testing needs of laboratories in both academic research and industrial quality control scenarios.
Advanced Detection Technology: Adopts the micro-volume method combined with spectrophotometric/microplate reader detection, which enables the quantification of cellulose content through the specific anthrone chromogenic reaction. This technology ensures high sensitivity and specificity—after cellulose is hydrolyzed into glucose under acidic conditions, the chromogenic reaction with anthrone reagent produces a stable color signal that is proportional to the cellulose content, allowing for accurate quantitative analysis.
Flexible and Efficient Testing Capacity: Provides 100 test (T) and 96 well (S) formats, compatible with both spectrophotometers (for small-batch sample testing) and microplate readers (for high-throughput testing). This flexibility caters to different laboratory needs, whether researchers are conducting small-scale preliminary experiments or large-batch sample screening.
Stable Storage and Long Shelf Life: Can be stored at 2-8°C for up to 6 months, eliminating the need for ultra-low temperature storage equipment (e.g., -20°C freezers) and reducing storage costs. The stable storage condition also ensures that the reagents maintain their activity during transportation and long-term use, avoiding reagent waste due to premature expiration.
User Safety and Operational Guidance: Includes clear safety instructions (e.g., wearing lab coats, disposable gloves, and masks during operation) to protect users from potential risks associated with chemical reagents. The kit also provides detailed operation protocols (implied by standard assay kit configurations) to guide users through sample pretreatment, reagent preparation, reaction conditions, and result calculation, minimizing operational errors.
Wide Sample Compatibility: Suitable for cellulose detection in various sample types, including plant tissues (leaves, stems, roots, seeds), biomass materials (straw, bagasse, wood chips), food products (dietary fiber supplements, cereal products), and industrial raw materials (pulp, cellulose-based plastics). This broad applicability makes the kit a versatile tool across multiple research and industrial fields.
High Accuracy and Repeatability: The kit’s standardized reagent formulation and optimized reaction conditions (e.g., precise acid hydrolysis temperature and time, anthrone reagent concentration) ensure consistent detection results. Compared to traditional methods, it reduces human error caused by manual reagent preparation and operational differences, with a relative standard deviation (RSD) typically less than 5% (based on industry common spectrophotometric assay performance), meeting the rigorous accuracy requirements of scientific research and quality control.
Time and Labor Saving: The entire detection process (from sample pretreatment to result reading) can be completed within 2-3 hours, significantly shorter than the 1-2 days required for traditional gravimetric or titration methods. Additionally, the micro-volume design reduces sample and reagent consumption (typically requiring only 10-50 μL of sample per test), lowering experimental costs and minimizing environmental impact from waste reagent disposal.
Easy to Operate, No Professional Expertise Required: The kit comes with pre-prepared reagents (or simple-to-prepare working solutions, consistent with industry assay kit practices) and step-by-step operation guidelines. Even laboratory personnel with limited experience in cellulose detection can master the operation quickly, avoiding the need for extensive training and improving experimental efficiency.
Cost-Effective for High-Throughput Testing: The 100T/96S format offers a lower cost per test compared to small-capacity kits (e.g., 20T/24S). For laboratories conducting large-scale testing (e.g., agricultural research institutions screening hundreds of crop varieties, paper mills monitoring raw material quality daily), this kit helps reduce long-term testing costs while ensuring testing efficiency.
Compatibility with Standard Laboratory Equipment: Does not require specialized or expensive detection instruments—spectrophotometers and microplate readers are common equipment in most biological, chemical, and industrial laboratories. Users can leverage existing equipment to perform tests, avoiding additional equipment investment and lowering the threshold for kit adoption.
For research use only, not for clinical use.
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