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| Product Name | Caspase-9 Colorimetric Assay Kit |
| Catalog No. | RESK-0182 |
| Description | Caspase-9, also known as ICE-LAP6 or Mch6, is activated by forming a complex with cytochrome c and Apaf-1, and further activates caspase-3, the most critical enzyme of apoptosis, thereby triggering the apoptotic cascade. Caspase-9 is an important upstream caspase in apoptotic signal transduction, and its activation can be regulated by phosphorylation. |
| Intended Use | For the detection of Caspase-9 activity in mammalian tissues and cells; suitable for studying Caspase-9-mediated apoptotic signaling pathways. |
| Principle / Technology | The assay is based on the specific hydrolysis of the peptide substrate LEHD-pNA (Leu-Glu-His-Asp-p-nitroanilide) by Caspase-9. The released free p-nitroaniline (pNA) shows maximum absorbance at 405 nm, and Caspase-9 activity is determined by colorimetric measurement of pNA. |
| Detection Method | Colorimetric assay (visible spectrophotometry); absorbance read at 405 nm |
| Sample Type | Mammalian tissues and cells |
| Performance Range / Specifications | Caspase-9 activity is determined by visible-light colorimetric measurement of pNA. Suitable for the detection of Caspase-9 activity in mammalian tissues and cells. |
| Specificity | Based on the specific hydrolysis of the LEHD-pNA substrate by Caspase-9. |
| Storage Conditions | Part I: store at 2-8 °C Part II: store at -25 to -15 °C |
| Shelf Life | 1 year |
| Package Specifications | 20 T 48 T 96 T |
| Expiration Date / Stability | Stable for 1 year when stored under the recommended conditions |
| Regulatory / Compliance | For research use only. |
| Recognition Site | LEHD (Leu-Glu-His-Asp) |
For research use only, not for clinical use.
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