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| Product Name | Caspase-8 Colorimetric Assay Kit |
| Catalog No. | RESK-0181 |
| Description | Caspases are a family of proteases involved in apoptosis, comprising more than ten members. Caspase-8, also known as FLICE, MACH or Mch5, normally exists as a zymogen and is activated upon apoptosis; it is regarded as an upstream caspase in apoptotic signal transduction. During Fas-receptor- and TNFR-1-mediated apoptosis, caspase-8 is activated to form a dimer, which in turn activates the downstream caspase-4, -6, -9 and -10. |
| Intended Use | For the detection of Caspase-8 activity in mammalian tissues and cells; suitable for studying Caspase-8 activity in apoptotic signaling pathways. |
| Principle / Technology | The assay is based on the specific hydrolysis of the peptide substrate Ile-Glu-Thr-Asp-p-nitroanilide (IETD-pNA) by Caspase-8. The released free p-nitroaniline (pNA) shows maximum absorbance at 405 nm, and Caspase-8 activity is determined by colorimetric measurement of pNA. |
| Detection Method | Colorimetric assay (visible spectrophotometry); absorbance read at 405 nm |
| Sample Type | Mammalian tissues and cells |
| Performance Range / Specifications | Caspase-8 activity is determined by visible-light colorimetric measurement of pNA. Suitable for the detection of Caspase-8 activity in mammalian tissues and cells. |
| Specificity | Based on the specific hydrolysis of the IETD-pNA substrate by Caspase-8. |
| Storage Conditions | Part I: store at 2-8 °C Part II: store at -25 to -15 °C |
| Shelf Life | 1 year |
| Package Specifications | 20 T 48 T 96 T |
| Expiration Date / Stability | Stable for 1 year when stored under the recommended conditions |
| Regulatory / Compliance | For research use only. |
| Recognition Site | IETD (Ile-Glu-Thr-Asp) |
For research use only, not for clinical use.
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