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| Product Name | Caspase-3 Colorimetric Assay Kit |
| Catalog No. | RESK-0177 |
| Description | Caspase-3 is the principal terminal protease in apoptosis and the most extensively studied caspase. It activates pro-caspase-2, -6, -7 and -9, and specifically hydrolyzes multiple key apoptotic proteins such as PARP, mediating chromatin condensation, apoptotic body formation and nuclear DNA fragmentation. Because Caspase-7 also uses DEVD-pNA as a substrate, this kit can be used to detect Caspase-3/7 activity. |
| Intended Use | For the detection of Caspase-3 (and Caspase-3/7) activity in mammalian tissues and cells; suitable for apoptosis research. |
| Principle / Technology | The assay is based on the specific hydrolysis of the peptide substrate DEVD-pNA (Asp-Glu-Val-Asp-p-nitroanilide) by Caspase-3. The released free p-nitroaniline (pNA) shows maximum absorbance at 405 nm, and Caspase-3 activity is determined by colorimetric measurement of pNA. |
| Detection Method | Colorimetric assay (visible spectrophotometry); absorbance read at 405 nm |
| Sample Type | Mammalian tissues and cells |
| Performance Range / Specifications | Caspase-3 activity is determined by visible-light colorimetric measurement of pNA. Suitable for the detection of Caspase-3 activity in mammalian tissues and cells. |
| Specificity | Based on the specific hydrolysis of the DEVD-pNA substrate by Caspase-3. Because Caspase-7 also hydrolyzes DEVD-pNA, this kit can be used to detect Caspase-3/7 activity. |
| Storage Conditions | Part I: store at 2-8 °C Part II: store at -25 to -15 °C |
| Shelf Life | 1 year |
| Package Specifications | 20 T 48 T 96 T |
| Expiration Date / Stability | Stable for 1 year when stored under the recommended conditions |
| Regulatory / Compliance | For research use only. |
| Recognition Site | DEVD (Asp-Glu-Val-Asp) |
For research use only, not for clinical use.
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