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| Product Name | Caspase-1 Colorimetric Assay Kit |
| Catalog No. | RESK-0175 |
| Description | Caspases are a family of proteases involved in apoptosis, comprising more than ten members. Caspase-1 is the only caspase that cleaves the precursors of IL-1beta and IL-18 to generate the active cytokines. Caspase-11 cleaves the 45 kD pro-caspase-1 to produce 20 kD and 10 kD fragments, which form a heterodimer and further assemble into an enzymatically active tetramer. Caspase-1 regulates apoptosis by cleaving Bcl-XL and modulates cytokine-mediated immune and inflammatory responses through cleavage of cytokine precursors. |
| Intended Use | For the detection of Caspase-1 activity in mammalian tissues and cells; suitable for apoptosis research. |
| Principle / Technology | The assay is based on the specific hydrolysis of the peptide substrate Tyr-Val-Ala-Asp-p-nitroanilide (YVAD-pNA) by Caspase-1. The released free p-nitroaniline (pNA) shows maximum absorbance at 405 nm, and Caspase-1 activity is determined by colorimetric measurement of pNA. |
| Detection Method | Colorimetric assay (visible spectrophotometry); absorbance read at 405 nm |
| Sample Type | Mammalian tissues and cells |
| Performance Range / Specifications | Caspase-1 activity is determined by visible-light colorimetric measurement of pNA. Suitable for the detection of Caspase-1 activity in mammalian tissues and cells. |
| Specificity | Based on the specific hydrolysis of the YVAD-pNA substrate by Caspase-1. |
| Storage Conditions | Part I: store at 2-8 °C Part II: store at -25 to -15 °C |
| Shelf Life | 1 year |
| Package Specifications | 20 T 48 T 96 T |
| Expiration Date / Stability | Stable for 1 year when stored under the recommended conditions |
| Regulatory / Compliance | For research use only. |
| Recognition Site | YVAD (Tyr-Val-Ala-Asp) |
For research use only, not for clinical use.
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