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| Product Name | Carnitine Palmitoyltransferase I (CPT-I) Activity Assay Kit, 50T/48S, 100T/96S |
| Catalog No. | FAMAK-YJL-0058 |
| Detection Method | Micro-volume method |
| Storage | Store at -20°C, 6 months |
| Intended Use | For research use only. |
| Note | For your safety and health, please wear lab coat, disposable gloves and mask during operation. |
| Product Introduction | Carnitine palmitoyltransferase I (CPT-I) is the key regulatory enzyme located on the outer mitochondrial membrane that catalyzes the transfer of a long-chain acyl group from acyl-CoA to carnitine, forming acylcarnitine. This is the committed and rate-limiting step for the entry of long-chain fatty acids into the mitochondria for β-oxidation. CPT-I activity is tightly regulated by malonyl-CoA (a potent inhibitor) and is central to understanding metabolic flexibility, insulin resistance, and disorders of fatty acid oxidation. This kit measures the forward CPT-I reaction by detecting the CoA-SH released. |
| Product Application | • Research on fatty acid oxidation, energy homeostasis, and metabolic flexibility. • Studies of insulin resistance, type 2 diabetes, and non-alcoholic fatty liver disease (NAFLD). • Investigation of genetic disorders of fatty acid metabolism (e.g., CPT-I deficiency). • Screening for compounds that modulate CPT-I activity as potential therapeutics. |
| Product Advantages | • Direct Measurement of Rate-Limiting Step: Provides functional data on the critical control point of long-chain fatty acid catabolism. • Malonyl-CoA Sensitivity: The assay can be performed in the presence or absence of malonyl-CoA to study the physiological regulation of CPT-I activity. • Optimized for Mitochondrial Preparations: Specifically designed for use with isolated mitochondria or permeabilized cells to maintain the enzyme's native membrane environment. • Sensitive Thiol Detection: Utilizes a sensitive colorimetric method (e.g., DTNB) to quantify the CoA-SH product, enabling accurate activity determination. |
For research use only, not for clinical use.
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