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| Product Name | Calcein AM Cell Activity Assay Kit (CCK-F) |
| Catalog No. | REKI-0036 |
| Description | Fluorescence-based cell activity kit built on Calcein AM, a membrane-permeant esterase substrate. Living cells hydrolyze the acetoxymethyl ester, trapping the strongly green fluorescent calcein inside, so the signal scales with intracellular esterase activity and membrane integrity. The kit is offered in 100-test, 500-test and 2500-test sizes, and unusually for fluorescence reagents it keeps for 60 months at -20 °C, which suits long-running screening programs. |
| Intended Use | Fluorescent measurement of cell activity through intracellular esterase function |
| Principle / Technology | Live cells convert membrane-permeant Calcein AM into green fluorescent calcein through intracellular esterases |
| Specificity | 100 T / 500 T / 2500 T; green fluorescence readout; 60 month shelf life |
| Reaction Conditions / Protocol | Add the Calcein AM working solution to the culture, incubate protected from light and record green fluorescence on a plate reader or microscope |
| Storage Conditions | -20 °C |
| Package Specifications | 100 T / 500 T / 2500 T |
| Key Features | Esterase-activity readout with intact-membrane requirement; three pack sizes up to 2500 tests; 60 month shelf life |
For research use only, not for clinical use.
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