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| Product Name | Bovine T3 (Triiodothyronine) ELISA Kit |
| Catalog No. | HTK-YJL-0403 |
| Synonyms | T3 ELISA Kit, Triiodothyronine ELISA Kit |
| Species | Bovine |
| Sample Type | Serum, plasma, cell culture supernatant, cell or tissue lysate, other liquid samples |
| Detection Method | Competitive ELISA, Coated with Antigen |
| Detection Wavelength | OD450 |
| Reaction Duration | 2 hours |
| Range | 0.156-10 ng/ml |
| Sensitivity | 0.094 ng/ml |
| Storage | 2-8°C (Sealed). Don't cryopreserve. |
| Specificity | Specifically binds with T3, no obvious cross reaction with other analogues. |
| Intended Use | For research use only. |
Triiodothyronine (T3) is one of the two primary active thyroid hormones circulating in the bloodstream of bovine (Bos taurus), with the other being thyroxine (T4). In bovine physiology, only about 20% of T3 is directly secreted by the thyroid gland; the majority (approximately 80%) is generated through peripheral conversion of T4 via deiodinase enzymes, primarily in the liver and kidneys of cattle. This hormone plays a critical role in regulating key physiological processes in bovines, including metabolism—by increasing oxygen consumption and heat production across nearly all tissues—and supporting normal growth, neurological development, and overall metabolic homeostasis.In research settings, accurate quantification of bovine T3 is essential for studying thyroid function, evaluating the impact of environmental factors (e.g., nutrition, stress) on bovine endocrine health, and investigating the role of thyroid hormones in bovine growth and productivity. Traditional methods for T3 detection often lack specificity or sensitivity, making ELISA (Enzyme-Linked Immunosorbent Assay) kits the preferred tool for researchers. The Bovine T3 (Triiodothyronine) ELISA Kit (Cat.No: HTK-YJL-0403) is designed to address this need, providing a reliable and efficient way to measure T3 levels in various bovine samples, thereby supporting advancements in veterinary research and animal science.
Utilizes a competitive ELISA method with antigen-coated microplates, ensuring high specificity for bovine T3 by minimizing cross-reactivity with other thyroid hormone analogues (e.g., T4) or non-target substances in samples.
Offers a detection range of 0.156–10 ng/ml, which aligns with the typical physiological concentration of T3 in bovine biological samples, enabling accurate measurement of both low and moderate T3 levels.
Boasts a high sensitivity of 0.094 ng/ml, allowing researchers to detect even trace amounts of T3 in samples, which is critical for studies involving subtle changes in thyroid hormone levels (e.g., response to dietary adjustments).
Requires a short reaction duration of 2 hours from sample addition to result reading, reducing experimental time and improving workflow efficiency for research laboratories.
Supports multiple bovine sample types, including serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples, providing flexibility for diverse research designs (e.g., in vitro cell studies, in vivo tissue analysis).
Ensures stable storage at 2–8°C (sealed) without the need for cryopreservation, simplifying inventory management and reducing the risk of reagent degradation during storage.
High Specificity: Specifically binds to bovine T3 with no obvious cross-reactivity with other thyroid analogues or interfering substances, ensuring that test results accurately reflect true T3 concentrations in samples.
Reliable Sensitivity: With a sensitivity of 0.094 ng/ml, the kit outperforms some competing bovine T3 ELISA kits (e.g., those with sensitivities of 0.16 ng/ml or 0.5 ng/ml) in detecting low T3 levels, making it suitable for studies requiring high precision.
Time-Efficient: The 2-hour reaction duration is shorter than the incubation time of many similar kits (which can range from 75 minutes to 5 hours), helping researchers complete experiments faster and handle more samples in a single workflow.
Sample Versatility: Compatibility with multiple sample types eliminates the need for researchers to purchase separate kits for different sample sources, reducing costs and streamlining experimental setup.
Easy Storage: Storage at 2–8°C (no cryopreservation required) avoids the need for specialized equipment (e.g., -20°C freezers) and minimizes reagent damage from repeated freeze-thaw cycles, extending the usable life of the kit.
Research-Focused Design: Optimized for bovine samples specifically, ensuring that the kit’s performance (e.g., detection range, sensitivity) is tailored to the unique physiological characteristics of bovine T3, unlike “universal” T3 kits that may not be calibrated for bovine samples.
For research use only, not for clinical use.
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