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| Product Name | Alcohol Dehydrogenase (ADH) Activity Assay Kit, 50T/48S |
| Catalog No. | FAMAK-YJL-0054 |
| Detection Method | UV spectrophotometry, Spectrophotometer |
| Storage | Store at -20°C, 6 months |
| Intended Use | For research use only. |
| Note | For your safety and health, please wear lab coat, disposable gloves and mask during operation. |
Alcohol Dehydrogenase (ADH) is a key oxidoreductase enzyme that plays a critical role in the metabolism of alcohols across prokaryotic and eukaryotic organisms. Its primary biological function involves catalyzing the reversible oxidation of primary alcohols to their corresponding aldehydes, coupled with the reduction of coenzymes such as nicotinamide adenine dinucleotide (NAD⁺) to NADH. This enzymatic reaction is not only fundamental to cellular energy metabolism—especially in tissues like the liver, where ADH detoxifies ethanol—but also serves as a vital marker in various research fields, including biochemistry, molecular biology, diagnostics, and environmental science.
In research settings, the accurate measurement of ADH activity is essential for multiple applications:
Biochemical Pathway Studies: Investigating the regulation of alcohol metabolism pathways in model organisms (e.g., yeast, mice) to understand how genetic or environmental factors impact enzyme function.
Research: Evaluating ADH activity in biological samples (e.g., serum, liver tissue homogenates) to explore its correlation with conditions like liver disease, alcohol dependence, or metabolic disorders. For instance, altered ADH isoenzyme levels have been linked to increased susceptibility to alcoholic liver injury.
Industrial Biotechnology: Monitoring ADH activity in microbial fermentation processes (e.g., ethanol production from biomass) to optimize yields and ensure process efficiency.
Environmental Monitoring: Detecting ADH in microorganisms isolated from polluted environments (e.g., soil, water contaminated with alcohols or aldehydes) to assess microbial detoxification capacity and environmental health.
Traditional methods for measuring ADH activity often relied on cumbersome techniques such as radioisotope labeling or colorimetric assays with low sensitivity. However, modern UV spectrophotometric assays—like the one enabled by this ADH Activity Assay Kit—have become the gold standard due to their simplicity, speed, and reliability. By detecting the absorbance change of NADH at 340 nm (a wavelength specific to the reduced coenzyme), this kit allows researchers to quantify ADH activity directly, eliminating the need for hazardous reagents or complex sample preparation steps. This advancement has significantly streamlined data collection in labs worldwide, making it easier to generate consistent, reproducible results for both basic and applied research projects.
High Compatibility with Diverse Sample Types: The kit is optimized to work with a wide range of biological and environmental samples, including tissue homogenates (liver, yeast, bacterial cultures), cell lysates (mammalian, insect, plant cells), serum, plasma, and even crude microbial extracts. This versatility eliminates the need for researchers to purchase multiple kits for different sample sources, reducing experimental costs and logistical hassle.
Robust UV Spectrophotometric Detection: Leverages the specific absorbance of NADH at 340 nm—an established, highly sensitive detection method—to ensure accurate quantification of ADH activity. Unlike colorimetric assays that may suffer from interference from sample pigments, UV-based detection minimizes background noise, leading to more precise and reliable data.
Ready-to-Use Reagent Formulation: All critical reagents (including substrate solutions, NAD⁺ coenzyme, and reaction buffers) are pre-mixed and pre-aliquoted in stable formulations. This design eliminates the need for time-consuming reagent preparation (e.g., weighing, dissolving, filtering) and reduces the risk of human error during setup, allowing researchers to start experiments within minutes of opening the kit.
Extended Storage Stability: When stored at -20°C as recommended, the kit maintains full activity for up to 6 months. Even after multiple freeze-thaw cycles (within reasonable limits), the reagents retain their performance, ensuring consistent results across experiments conducted over weeks or months—ideal for long-term research projects.
Flexible Reaction Scaling: The kit’s 50T/48S format (50 total reactions, 48 sample reactions) is designed to accommodate both small-scale pilot experiments and larger batch analyses. Researchers can easily adjust the reaction volume (with proportional reagent scaling) to fit different spectrophotometer microplate formats (e.g., 96-well, 384-well) or cuvette-based systems, adapting to varying lab equipment and experimental needs.
Superior Sensitivity and Detection Range: The assay can detect ADH activity as low as 0.01 U/mL (depending on sample type) and covers a broad linear range of 0.01–10 U/mL. This high sensitivity enables the measurement of low-abundance ADH in samples where the enzyme concentration is minimal (e.g., dilute cell lysates or serum from healthy individuals), while the wide linear range eliminates the need for excessive sample dilution—saving time and reducing the risk of dilution errors.
Exceptional Reproducibility and Precision: Each batch of reagents undergoes rigorous quality control testing, including inter-assay and intra-assay variability assessments. The intra-assay coefficient of variation (CV) is ≤5%, and the inter-assay CV is ≤8%, ensuring that results from the same sample are consistent across different experiments and different users. This reliability is critical for publishing research data and validating experimental findings.
Time and Labor Efficiency: The entire assay protocol can be completed in as little as 30 minutes (from sample preparation to data readout). Unlike traditional methods that require incubation times of several hours or overnight, this kit accelerates the experimental workflow, allowing researchers to process more samples in less time. Additionally, the simple protocol requires no specialized technical skills, making it accessible to both experienced researchers and lab technicians new to enzyme activity assays.
Cost-Effective per Reaction: The 50T/48S format provides a low cost per reaction compared to smaller kit sizes or single-use assays. For labs that conduct regular ADH activity measurements (e.g., core facilities, research teams with ongoing projects), this kit offers significant cost savings without compromising on quality. Furthermore, the stable reagent formulation reduces reagent waste, as unused portions can be stored for future use (within the 6-month shelf life).
Minimal Interference from Sample Components: The assay buffer is formulated with additives that inhibit non-specific reactions and reduce interference from common sample contaminants (e.g., proteins, lipids, reducing agents). This ensures that the measured absorbance change is specifically due to ADH-catalyzed NADH production, rather than background reactions from other enzymes or molecules in the sample. As a result, researchers can trust that their data accurately reflects true ADH activity.
For research use only, not for clinical use.
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