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| Product Name | Acidic Xylanase (ACX) Activity Assay Kit, 100T/48S |
| Catalog No. | SCAK-YJL-0163 |
| Detection Method | Micro-volume method, Spectrophotometer/Microplate Reader |
| Storage | Store at 2-8°C, 6 months |
| Intended Use | For research use only. |
| Note | For your safety and health, please wear lab coat, disposable gloves and mask during operation. |
Xylanase, classified under EC 3.2.1.8, is a key class of hydrolytic enzymes that specifically target and break down xylan—a major component of hemicellulose in plant cell walls. This enzyme plays a pivotal role in bridging biological processes and industrial applications, as it catalyzes the hydrolysis of xylan into smaller, usable molecules like xylose, reducing oligosaccharides, and monosaccharides.
Acidic Xylanase (ACX), a specialized variant of xylanase, is uniquely adapted to function in acidic environments—a trait that makes it indispensable in industries and research fields where low pH conditions are prevalent. Unlike neutral or alkaline xylanases, ACX is primarily isolated from acid-tolerant microorganisms, including specific strains of fungi, bacteria, and certain molds. These microbial sources endow ACX with the stability and activity needed to perform effectively in acidic settings, addressing a critical gap in processes that operate under low pH.
In industrial contexts, ACX has become a cornerstone in multiple sectors:
Brewing Industry: It decomposes the cell walls of raw materials (such as barley and wheat) and breaks down β-glucan, a polysaccharide that can increase the viscosity of brewing mashes. By reducing viscosity, ACX improves the flow of materials during processing, promotes the release of essential flavor compounds and fermentable sugars, and ultimately enhances the efficiency and quality of beer or other fermented beverages.
Feed Industry: Animal feeds often contain high levels of non-starch polysaccharides (NSPs), including xylan, which are indigestible by most livestock. ACX degrades these NSPs, reducing their anti-nutritional effects (such as interfering with nutrient absorption) and improving the digestibility of proteins, carbohydrates, and other nutrients. This leads to better feed conversion rates, faster animal growth, and reduced nutrient excretion.
Biofuel Production: In the production of second-generation biofuels (e.g., ethanol from lignocellulosic biomass), ACX helps break down the hemicellulose fraction of biomass (like corn stover, sugarcane bagasse, or wood chips). By converting xylan into fermentable sugars, it increases the overall yield of biofuels, making the process more economically viable and sustainable.
Paper Manufacturing: In pulp and paper production, ACX aids in the pre-treatment of wood pulp. It removes xylan from the pulp, which improves the brightness of paper, reduces the need for harsh chemical bleaching agents, and lowers the environmental impact of the manufacturing process.
In research, the demand for reliable ACX activity measurement has grown alongside its industrial importance. Accurate quantification of ACX activity is essential for:
Optimizing enzyme production processes (e.g., tuning microbial fermentation conditions to maximize ACX yield).
Evaluating enzyme performance in different acidic environments (e.g., testing how ACX behaves at varying pH levels in feed or brewing systems).
Ensuring quality control during enzyme formulation (e.g., verifying that ACX-containing products meet specified activity standards).
Advancing studies on microbial physiology and enzyme engineering (e.g., researching how to modify ACX for enhanced stability or activity).
Our Acidic Xylanase (ACX) Activity Assay Kit (100T/48S) is designed to meet this critical need. It provides a precise, efficient, and user-friendly method for measuring ACX activity, supporting both academic research and industrial quality assurance efforts.
Dual Detection Compatibility: The kit supports both micro-volume method and use with common laboratory instruments (spectrophotometer or microplate reader), offering flexibility to adapt to different laboratory setups—whether researchers need high-throughput testing (with a microplate reader) or small-sample analysis (with a spectrophotometer).
Stable Storage and Long Shelf Life: When stored at 2-8°C, the kit maintains its functionality for up to 6 months. This long shelf life reduces the need for frequent reordering, minimizes waste from expired reagents, and ensures consistent performance across multiple experiments or batches of testing.
Broad Sample Applicability: It is suitable for analyzing ACX activity in a wide range of sample types, including enzyme preparations (e.g., purified ACX samples), animal feed extracts, brewing process intermediates (e.g., mash or wort), and microbial culture supernatants (e.g., from ACX-producing fungi or bacteria).
Rapid and Reliable Assay Protocol: The assay leverages a well-validated colorimetric principle (based on the reaction between reducing sugars and 3,5-dinitrosalicylic acid in a boiling water bath), ensuring that results are both fast to obtain and consistent. Researchers can complete a full assay efficiently, without lengthy or complex sample pre-treatment steps.
Research-Focused Design: The kit is optimized for research use, with reagent quantities (100T/48S) that align with typical research workloads—providing enough tests for multiple experiments while avoiding excessive reagent storage or spoilage.
High Accuracy and Precision: The assay’s reliance on a 540 nm absorbance measurement (a wavelength with a characteristic peak for the color reaction product) ensures that even small changes in ACX activity are detected. This high sensitivity minimizes measurement errors, allowing researchers to obtain reliable data for comparing ACX activity across different samples or experimental conditions.
User Safety Priority: The kit includes clear safety guidelines (advising the use of lab coats, disposable gloves, and masks during operation), protecting users from potential reagent exposure and ensuring compliance with standard laboratory safety protocols.
Cost-Effective for Routine Testing: With 100 tests (100T) or 48 tests (48S) per kit, the product offers a cost-efficient solution for routine ACX activity measurement. It eliminates the need to purchase individual reagents (e.g., 3,5-dinitrosalicylic acid, xylan substrates) separately, reducing overall experimental costs and simplifying inventory management.
No Specialized Technical Expertise Required: The kit’s protocol is straightforward, with no need for advanced laboratory skills or specialized equipment beyond common instruments (spectrophotometer/microplate reader). This makes it accessible to researchers at all experience levels, from graduate students to seasoned scientists.
Consistent Performance Across Batches: Each kit is manufactured to strict quality standards, ensuring that reagent composition and activity are consistent across different production batches. This batch-to-batch consistency means results from one kit can be reliably compared to data from previous or future kits, supporting long-term research projects.
For research use only, not for clinical use.
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