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| Product Name | 6-Phosphogluconate Dehydrogenase (6PGDH) Activity Assay Kit, 60T/50S |
| Catalog No. | EATK-YJL-0029 |
| Testing Equipment | UV spectrophotometer |
| Number of Detectable Samples | 50 Samples |
| Testing Accessories | 1 mL Quartz cuvette (d=10 mm) |
| Estimated Measurement Time | 5 h (50 Samples) |
| Storage | Store according to label instructions |
| Reagents to be Prepared | None |
Enzyme Function & Metabolic Role: 6-Phosphogluconate Dehydrogenase (6PGDH) is a key enzyme in the pentose phosphate pathway (PPP), a critical metabolic route in cells. It specifically catalyzes the oxidative decarboxylation of 6-phosphogluconate to ribulose-5-phosphate, with a simultaneous reaction that reduces NADP⁺ to NADPH. This dual action makes 6PGDH essential for two core cellular processes: generating ribulose-5-phosphate (a precursor for nucleotide and nucleic acid synthesis) and producing NADPH (a vital reducing agent for biosynthetic reactions and cellular defense against oxidative stress).
Biological Significance in Research: 6PGDH activity is closely linked to cellular energy balance, growth rate, and viability. In stress physiology studies, for example, changes in 6PGDH activity can indicate how cells respond to oxidative or environmental stressors. Additionally, research has shown that 6PGDH plays a role in specialized biological contexts—such as maintaining redox homeostasis in red blood cells (where NADPH supports glutathione regeneration) and influencing tumor cell metabolism (inhibition of 6PGDH may reduce RNA biosynthesis and increase reactive oxygen species in tumor cells, supporting anti-cancer research).
Need for Reliable Assay Kits: Accurate measurement of 6PGDH activity is critical for advancing research in fields like cell biology, biochemistry, and disease physiology. Our 6PGDH Activity Assay Kit is designed to meet this need, enabling researchers to quantify enzyme activity in biological samples with consistency and precision—supporting studies on metabolic pathways, stress responses, and disease-related enzyme dysregulation.
Enables quantitative measurement of 6PGDH activity in biological samples, providing objective and reproducible data for research experiments.
Comes with all necessary reagents (no additional reagent preparation required), simplifying experimental setup and reducing the risk of errors from manual reagent mixing.
Includes a 1 mL quartz cuvette (d=10 mm) as a testing accessory, eliminating the need for researchers to source compatible cuvettes separately and ensuring immediate use with UV spectrophotometers.
Designed to process up to 50 samples per kit (60T/50S format), making it suitable for both small-scale experiments and medium-throughput research projects.
Offers clear storage guidelines (follow label instructions) to maintain reagent stability, preserving assay performance over time when stored correctly.
Compatible with UV spectrophotometers—a widely available piece of laboratory equipment—ensuring accessibility for most research laboratories without the need for specialized or high-cost instruments.
User-Friendly Workflow: With no reagents to prepare and included testing accessories, the kit minimizes hands-on time and technical complexity, making it suitable for researchers of all skill levels (from early-career scientists to experienced technicians).
Time-Efficient Assay Process: The estimated measurement time for 50 samples is 5 hours, balancing thoroughness with efficiency—allowing researchers to complete experiments within a typical workday without compromising data quality.
Broad Sample Compatibility: While optimized for common research samples (e.g., tissue homogenates, cell lysates, serum/plasma, and bacterial cultures), the kit’s flexible design adapts to various biological sample types, supporting diverse research objectives (e.g., animal, plant, or microbial biology studies).
Consistent and Trustworthy Results: The assay’s reliance on UV spectrophotometry (a well-validated detection method for NADPH-related reactions) ensures high accuracy, with minimal variability between replicate measurements—critical for reliable data analysis and publication-quality results.
Cost-Effective for Research Budgets: The 60T/50S format provides a balanced sample-to-cost ratio, offering value for money while avoiding waste from oversized kits (ideal for laboratories with moderate sample volumes).
For research use only, not for clinical use.
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