| Product Name | 6-Phosphogluconate Dehydrogenase (6PGDH) Activity Assay Kit, 110T/100S |
| Catalog No. | EATK-YJL-0045 |
| Testing Equipment | Microplate reader |
| Number of Detectable Samples | 100 Samples |
| Testing Accessories | 96-well UV plate |
| Estimated Measurement Time | 5 h (100 Samples) |
| Storage | Store according to label instructions |
| Reagents to be Prepared | None |
| Product Introduction | 6-Phosphogluconate dehydrogenase (6-PGDH) is a key enzyme in the pentose phosphate pathway, playing a critical role in cellular NADPH regeneration. NADPH levels are essential for maintaining redox balance, supporting energy metabolism, and promoting cell growth and viability. This assay kit offers a simple, sensitive, and efficient method for measuring 6-PGDH activity. The enzyme catalyzes the conversion of 6-phosphogluconate and NADP⁺ to generate NADPH, which then reacts with a specialized colorimetric probe to produce a colored compound. The rate of color formation, measured spectrophotometrically, is directly proportional to 6-PGDH activity, enabling accurate and quantitative evaluation. |
| Product Application | Quantifying 6-PGDH activity for pentose phosphate pathway studies, metabolic disorder research, and enzymology investigations. |
| Product Advantages | • High Sensitivity: Precisely detects low enzymatic activity levels through NADPH generation • Optimized Protocol: Streamlined procedure reduces handling time and minimizes operational errors • Broad Sample Compatibility: Validated for cell lysates, tissue extracts, and bacterial samples • Reproducible Results: Ensures high consistency across replicates and batches |
For research use only, not for clinical use.
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