- Home
- IVD
- By Technology Types
- By Diseases Types
- By Product Types
- Research
- Resource
- Distributors
- Company
| Product Name | 25-HVD3 (25-Hydroxy Vitamin D3) ELISA Kit, 0.781-50 ng/ml |
| Catalog No. | HTK-YJL-0373 |
| Synonyms | 25-HVD3 ELISA Kit, 25-Hydroxy Vitamin D3 ELISA Kit |
| Species | Universal |
| Sample Type | Serum, plasma, cell culture supernatant, cell or tissue lysate, other liquid samples |
| Detection Method | Competitive ELISA, Coated with Antigen |
| Detection Wavelength | OD450 |
| Reaction Duration | 2 hours |
| Range | 0.781-50 ng/ml |
| Sensitivity | 0.469 ng/ml |
| Storage | 2-8°C (Sealed). Don't cryopreserve. |
| Specificity | Specifically binds with 25-HVD3, no obvious cross reaction with other analogues. |
| Intended Use | For research use only. |
25-Hydroxy Vitamin D3 (25-HVD3), also widely known as calcidiol or calcifediol, is a critical intermediate in the vitamin D activation pathway—serving as the gold-standard biomarker for evaluating an individual’s or organism’s vitamin D status in both clinical research and basic life science studies. Its biological formation and functional role make it indispensable for understanding multiple physiological processes and disease-related mechanisms.
Biosynthesis Pathway: 25-HVD3 is produced through a key hydroxylation step in the liver. Specifically, vitamin D3 (cholecalciferol)—obtained either from dietary intake (e.g., fatty fish, fortified dairy products) or cutaneous synthesis via sunlight exposure—is converted by the enzyme cholecalciferol 25-hydroxylase (CYP2R1) located in the endoplasmic reticulum of hepatocytes. This conversion is a rate-limiting step in vitamin D metabolism, as 25-HVD3 is the primary form of vitamin D stored in the body and the precursor to the active hormone.
Conversion to Active Hormone: After synthesis in the liver, 25-HVD3 is transported to the kidneys, where it undergoes a second hydroxylation reaction catalyzed by 25-hydroxyvitamin D3 1-α-hydroxylase (CYP27B1) to form calcitriol (1,25-(OH)₂D₃)—the biologically active form of vitamin D. Calcitriol regulates critical physiological functions, including intestinal calcium and phosphorus absorption, renal calcium reabsorption, and bone mineralization. In some tissues (e.g., skin, immune cells), local conversion of 25-HVD3 to calcitriol also supports tissue-specific functions like immune modulation and cell differentiation.
Clinical and Research Significance: The concentration of 25-HVD3 in biological samples (such as serum or plasma) is widely recognized as the most sensitive and reliable indicator of vitamin D status. Abnormal 25-HVD3 levels are closely linked to various health and research endpoints:
Low 25-HVD3 concentrations (often defined as <80 nmol/L in many studies) are associated with conditions like osteoporosis (due to impaired bone mineralization), vitamin D malabsorption (e.g., in celiac disease or inflammatory bowel disease), and chronic kidney disease (where reduced CYP27B1 activity limits calcitriol production).
In research fields such as nutrition metabolism, bone biology, and reproductive science, measuring 25-HVD3 helps investigate the role of vitamin D in processes like fetal development, age-related bone loss, and metabolic disorders (e.g., type 2 diabetes).
Need for Accurate Detection: Given its central role in vitamin D metabolism and disease association, precise quantification of 25-HVD3 is essential for advancing research in fields like endocrinology, geriatrics, and public health. Traditional detection methods (e.g., immunoassays with low sensitivity) often fail to capture subtle changes in 25-HVD3 levels, highlighting the need for reliable, high-performance assay kits like the 25-HVD3 ELISA Kit (Cat.No: HTK-YJL-0373).
Broad Species Compatibility: Designed as a universal kit, it is compatible with samples from multiple species (not limited to humans), enabling researchers to conduct cross-species studies in fields like comparative biology, veterinary research, and animal models of human disease (e.g., mouse or rat models of osteoporosis).
Diverse Sample Type Support: This kit accommodates a wide range of biological samples, including serum, plasma, cell culture supernatant, and cell/tissue lysate. This flexibility eliminates the need for researchers to purchase separate kits for different sample types, streamlining experimental workflows and reducing costs.
Competitive ELISA with Antigen Coating: Utilizes a competitive ELISA format with pre-coated antigen, a well-validated technique for small-molecule analytes like 25-HVD3. The pre-coated antigen ensures consistent assay conditions across wells and batches, minimizing variability and improving data reproducibility.
Optimal Detection Wavelength: Calibrated for detection at 450 nm—a standard wavelength in most laboratory microplate readers. This compatibility means researchers do not need specialized equipment, ensuring easy integration into existing lab setups.
Rapid Assay Time: The total reaction duration is only 2 hours, significantly shorter than many conventional ELISA kits (which often require 3–4 hours). This reduces experimental turnaround time, allowing researchers to process more samples or complete studies faster.
Controlled Storage Conditions: Requires storage at 2–8°C (sealed, no cryopreservation). This storage requirement is straightforward to maintain in standard laboratory refrigerators, avoiding the need for expensive ultra-low temperature freezers and preserving kit stability over time.
Excellent Sensitivity: With a sensitivity of 0.469 ng/ml, this kit can detect low concentrations of 25-HVD3 that may be missed by less sensitive assays (e.g., kits with sensitivity >1 ng/ml). This is critical for research investigating subtle changes in vitamin D status, such as early-stage vitamin D deficiency or minor fluctuations in response to dietary interventions.
Wide Detection Range: Covers a range of 0.781–50 ng/ml, which aligns with the physiological range of 25-HVD3 in most biological samples (e.g., normal serum 25-HVD3 levels are typically 20–150 nmol/L, or ~8–60 ng/ml). This eliminates the need for excessive sample dilution (which can introduce errors) and ensures accurate quantification for both normal and abnormal samples.
High Specificity: Specifically binds to 25-HVD3 with no obvious cross-reactivity with other vitamin D analogues (e.g., vitamin D2, calcitriol, or 24-hydroxycalcidiol). This specificity prevents false-positive or false-negative results, ensuring that measured values accurately reflect 25-HVD3 concentrations.
Research-Grade Reliability: Manufactured to meet strict research standards, the kit ensures consistent performance across batches. This reliability is critical for longitudinal studies or multi-laboratory collaborations, where data comparability is essential.
User-Friendly Protocol: The kit’s assay workflow is designed to be simple and easy to follow, even for researchers with limited ELISA experience. Clear step-by-step instructions (included in the datasheet) reduce the risk of operational errors, making it accessible for labs of all sizes.
For research use only, not for clinical use.
|
There is no product in your cart. |
Copyright © 2025 Alta DiagenoTech. All rights reserved.
