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| Product Name | 25-HVD2 (25-Hydroxy Vitamin D2) ELISA Kit |
| Catalog No. | HTK-YJL-0485 |
| Synonyms | 25-HVD2 ELISA Kit, 25-Hydroxy Vitamin D2 ELISA Kit |
| Species | Universal |
| Sample Type | Serum, plasma, cell culture supernatant, cell or tissue lysate, other liquid samples |
| Detection Method | Competitive ELISA, Coated with Antigen |
| Detection Wavelength | OD450 |
| Reaction Duration | 2 hours |
| Range | 0.781-50 ng/ml |
| Sensitivity | 0.469 ng/ml |
| Storage | 2-8°C (Sealed). Don't cryopreserve. |
| Specificity | Specifically binds with 25-HVD2, no obvious cross reaction with other analogues. |
| Intended Use | For research use only. |
• Vitamin D plays a pivotal role in maintaining calcium homeostasis, bone health, and immune system regulation. Among its various forms, 25-hydroxyvitamin D2 (25(OH)D2) is a critical metabolite used to assess vitamin D2 status in research studies. This metabolite is primarily derived from dietary sources such as fortified foods, supplements, and certain fungi, distinguishing it from vitamin D3, which is synthesized in the skin upon exposure to sunlight.
• Researchers studying vitamin D metabolism, nutritional deficiencies, or the effects of supplementation rely on accurate quantification of 25(OH)D2 to understand its physiological and pathological implications. The 25-Hydroxy Vitamin D2 ELISA Kit is designed to meet this need by providing a sensitive, reproducible, and high-throughput method for detecting 25(OH)D2 in biological samples like serum, plasma, or cell culture supernatants.
• This kit supports studies in endocrinology, nutrition, immunology, and chronic disease research, enabling scientists to explore correlations between vitamin D2 levels and health outcomes such as bone density, autoimmune disorders, or cardiovascular health. Its compatibility with standard laboratory equipment makes it accessible for both small-scale and large-scale research projects.
• High Sensitivity: Detects low concentrations of 25(OH)D2 (typically ≤ 1 ng/mL), ensuring accurate results even in samples with minimal metabolite presence.
• Wide Detection Range: Covers a broad dynamic range (e.g., 0.5–100 ng/mL), reducing the need for sample dilution and minimizing errors.
• Reproducibility: Consistent inter- and intra-assay precision (CV < 10%) guarantees reliable data across multiple experiments.
• User-Friendly Protocol: Simplified steps with pre-coated plates, ready-to-use reagents, and clear instructions reduce hands-on time and training requirements.
• Compatibility: Works with common sample types, including human serum, plasma, and cell lysates, without requiring specialized pretreatment.
• Short Incubation Time: Results are obtained within 2–4 hours, accelerating research workflows compared to traditional methods like HPLC or LC-MS.
• Cost-Effective: Lower per-sample cost compared to instrumental techniques like mass spectrometry, making it ideal for large-scale studies.
• Scalability: Suitable for both small pilot studies and high-throughput screening, with 96-well plate formats supporting up to 40 samples in duplicate.
• Stability: Reagents are stable at 2–8°C for 12 months, minimizing waste and ensuring consistent performance over time.
• No Cross-Reactivity: High specificity for 25(OH)D2 with minimal interference from 25(OH)D3 or other vitamin D metabolites, ensuring accurate differentiation.
• Validation-Ready: Data provided with each kit includes standard curves, quality control ranges, and troubleshooting tips to streamline validation in research settings.
For research use only, not for clinical use.
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