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| Product Name | 2× Chemically Modified Hot-Start Taq Master Mix with Loading Dye |
| Catalog No. | MDKPK-0021 |
| Description | A ready-to-use 2× hot-start Taq PCR mix using chemical modification to suppress polymerase activity during reaction setup. Blue loading dye is included for direct gel analysis. |
| Intended Use | Hot-start PCR and identification of low-copy targets from complex templates. |
| Principle / Technology | Chemically modified Taq polymerase remains fully blocked below 80°C and is activated by heating at 95°C for 5 minutes. |
| Detection Method | Endpoint PCR followed by direct gel electrophoresis. |
| Sample Type | Genomic DNA, cDNA and plasmid DNA, including low-copy targets in complex templates. |
| Sensitivity / LOD | Demonstrated with serially diluted plasmid templates from 10−2 ng to 10−7 ng. |
| Specificity | Hot-start chemistry minimizes polymerase activity during setup and improves amplification specificity. |
| Reaction Conditions / Protocol | Use an initial 95°C activation step for 5 minutes before cycling. |
| Components / Formulation | 2× chemically modified hot-start Taq master mix with optimized buffer, stabilizers, electrophoresis buffer and blue loading dye. |
| Storage Conditions | Store at −20°C. |
| Package Specifications | 15 × 1 mL. |
| Product Form | 2× hot-start PCR master mix with loading dye. |
| Key Features | Complete activity blocking below 80°C; 95°C heat activation; low-copy target amplification; direct gel loading; ready-to-use format. |
| Compatibility | PCR products carry 3′-A overhangs and are suitable for T-vector cloning. |
For research use only, not for clinical use.
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