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| Product Name | Vitamin B5 HPLC Assay Kit, 50T/48S |
| Catalog No. | VMAK-YJL-0010 |
| Detection Method | High performance liquid chromatography, HPLC |
| Storage | Store at 2-8°C, 6 months |
| Intended Use | For research use only. |
| Note | For your safety and health, please wear lab coat, disposable gloves and mask during operation. |
Vitamin B5, also known as pantothenic acid, is a water-soluble B-vitamin that plays a critical role in multiple biological processes essential for life. As a key component of coenzyme A (CoA) and phosphopantetheine, it is deeply involved in fatty acid metabolism, including the oxidation of fatty acids and carbohydrates to produce adenosine triphosphate (ATP)—the primary energy source for cells. Additionally, Vitamin B5 contributes to the synthesis of vital molecules such as amino acids, ketones, cholesterol, phospholipids, and steroids, and supports porphyrin and hemoglobin biochemistry as well as normal adrenal function.
Given its widespread presence in foods and its significance in biological systems, accurate detection of Vitamin B5 is crucial for research applications—such as studying nutrient metabolism in biological samples, evaluating Vitamin B5 content in experimental formulations, or investigating the role of Vitamin B5 in cellular processes. Traditional detection methods for Vitamin B5 include microbiological assays, but high-performance liquid chromatography (HPLC) has emerged as a reliable choice for research use due to its ability to deliver precise and consistent quantitative results.
The Vitamin B5 HPLC Assay Kit is specifically designed to meet the needs of researchers seeking a robust tool for Vitamin B5 quantification. It leverages the HPLC technique, which separates and detects Vitamin B5 in samples with high specificity, making it suitable for analyzing various research samples (e.g., experimental food matrices, biological extracts used in research) where accurate Vitamin B5 measurement is required.
HPLC-Based Detection: Utilizes high-performance liquid chromatography, a well-established analytical technique known for high specificity and accuracy in separating and quantifying Vitamin B5, ensuring reliable results for research experiments.
Defined Test Capacity: Provides 50 tests (50T) with 48 sample positions (48S), offering a practical test volume to support medium-scale research projects, reducing the need for frequent kit reorders.
Controlled Storage Conditions: Requires storage at 2–8°C, a standard refrigerated temperature easily maintained in most research laboratories, and maintains stability for 6 months, allowing for flexible use over an extended period.
Safety-Focused Design: Includes a clear safety note recommending the use of lab coats, disposable gloves, and masks during operation, aligning with standard laboratory safety protocols to protect researchers.
Research-Only Optimization: Formulated exclusively for research purposes, with specifications tailored to meet the analytical needs of research applications (e.g., nutrient metabolism studies, experimental sample analysis) rather than clinical use.
High Result Reliability: HPLC technology minimizes interference from other sample components, ensuring that the quantified Vitamin B5 levels are accurate and reproducible—critical for validating research findings and enabling consistent experimental outcomes.
Laboratory Compatibility: The 2–8°C storage requirement and standard HPLC operation align with the equipment and infrastructure of most research laboratories, eliminating the need for specialized storage or additional costly instruments.
Cost-Effective Test Volume: The 50T/48S capacity balances test quantity and kit cost, providing researchers with a cost-efficient solution for multiple experiments without excessive waste of reagents.
Long Shelf Life: A 6-month shelf life allows laboratories to stock the kit in advance, avoiding last-minute shortages and supporting long-term research planning, especially for projects with staggered sample analysis timelines.
For research use only, not for clinical use.
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