- Home
- IVD
- By Technology Types
- By Diseases Types
- By Product Types
- Research
- Resource
- Distributors
- Company
Cat.No: PT-QCY-0018 Datasheet Instruction for Use
Specification Quantities
Price $829.41–1382.35
| Product Name | Strongyloides stercoralis IgG Rapid Test Kit |
| Catalog No. | PT-QCY-0018 |
| Description | This kit employs a double-antigen sandwich enzyme-linked immunosorbent assay (ELISA) to detect human Strongyloides stercoralis IgG antibodies (SS IgG) in specimens. The microplate is coated with purified antigen to create a solid-phase antigen that binds to the Strongyloides stercoralis IgG (SS IgG) in the sample. After washing to remove unbound antibodies and other components, the antigen binds to HRP-labeled antibody, forming an antigen-antibody-enzyme-labeled antigen complex. Following thorough washing, the substrate TMB is added to produce a color reaction. TMB is converted to blue under the catalysis of HRP and then to yellow under the action of acid. The absorbance (OD value) is measured at a wavelength of 450 nm using a microplate reader and compared with the cutoff value to determine the presence or absence of human Strongyloides stercoralis IgG antibodies (SS IgG) in the sample. |
| Kit Components | Sealing Film: 2 sheets (for 48 wells) Enzyme-labeled Coated Plate: 1×48 Negative Control: 0.5 ml × 1 bottle Positive Control: 0.5 ml × 1 bottle Enzyme-labeled Reagent: 3 ml × 1 bottle Sample Diluent: 3 ml × 1 bottle Chromogenic Agent A: 3 ml × 1 bottle Chromogenic Agent B: 3 ml × 1 bottle Stop Solution: 3 ml × 1 bottle Concentrated Wash Buffer: (20 ml × 20-fold) × 1 bottle |
| Sample Type | Human feces, serum, plasma, and related liquid samples |
| Sample Volume | 10 μL/well/sample |
| Assay Time | 15 minutes |
| Detection Wavelength | Single wavelength 450 nm, dual wavelengths 450 nm and 630 nm |
| Test Procedure | After equilibrating at room temperature for 20 minutes, remove the required strips; seal the remaining strips in a resealable bag and return them to 4°C. Set up negative and positive control wells and sample wells. Add 50 μL of negative control and 50 μL of positive control to the negative and positive control wells, respectively. For the sample wells, first add 10 μL of the sample to be tested, followed by 40 μL of sample diluent. Next, add 100 μL of horseradish peroxidase (HRP)-labeled detection antigen to each well (negative control, positive control, and sample wells). Cover the wells with a plate seal and incubate in a 37°C water bath or incubator for 60 minutes. Discard the liquid, blot dry with absorbent paper, fill each well with washing solution, let stand for 1 minute, shake off the washing solution, blot dry with absorbent paper, and repeat this washing procedure 5 times. Add 50 μL each of Chromogenic Agent A and B to each well, and incubate at 37°C in the dark for 15 minutes. Add 50 μL of stop solution to each well, and measure the OD value of each well at a wavelength of 450 nm within 15 minutes. |
| Results | Test Validity: The mean OD value of the positive control wells is ≥1.00; the mean OD value of the negative control wells is ≤0.15. Calculation of the Cut-off Value: Cut-off Value = Mean OD of the negative control wells + 0.15 Negative Result: If the sample OD is < the cut-off value, the sample is negative. Positive Result: If the sample OD is > the cut-off value, the sample is positive. |
| Storage and Transportation | Store unopened kits at 4°C. Once opened, store standards at -20°C and other components at 4°C. |
For research use only, not for clinical use.
|
There is no product in your cart. |
Copyright © 2026 Alta DiagnoTech. All rights reserved.
