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| Product Name | Soil Peroxidase (S-POD) Activity Assay Kit, 120T/50S |
| Catalog No. | EATK-YJL-0065 |
| Testing Equipment | Microplate reader |
| Number of Detectable Samples | 50 Samples |
| Testing Accessories | 96-well plate |
| Estimated Measurement Time | 2 h (50 Samples) |
| Storage | Store according to label instructions |
| Reagents to be Prepared | Ether |
Soil Peroxidase (S-POD) is a key redox enzyme widely present in soil ecosystems, primarily derived from the metabolic activities of soil microorganisms (such as bacteria, fungi, and actinomycetes). It plays a critical role in maintaining soil health and ecological balance by mediating multiple biogeochemical processes.
The core functional role of S-POD lies in its ability to catalyze the oxidation of various organic and inorganic substrates, including phenols, amines, and hydrocarbon oxidation products, with hydrogen peroxide (H₂O₂) as the electron acceptor. This catalytic reaction not only eliminates the toxic effects of harmful substances (e.g., excess H₂O₂, phenolic compounds, and aldehydes) in the soil but also promotes the formation and transformation of soil humus—an essential component for enhancing soil fertility, water retention, and nutrient availability.
From a research perspective, the activity of S-POD is a reliable biological indicator for evaluating soil quality. It directly reflects the intensity of soil respiration and the overall activity level of soil microbial communities. Changes in S-POD activity can signal shifts in soil environmental conditions (e.g., pollution, acidification, or nutrient deficiency), making it a focal point in studies related to soil ecology, environmental monitoring, agricultural sustainability, and remediation of contaminated soils.
The detection principle of this S-POD Activity Assay Kit is based on the enzyme’s catalytic 特性: S-POD catalyzes the oxidation of specific substrates to generate colored products (e.g., quinones) that exhibit a characteristic absorption peak at a specific wavelength. By measuring the change in absorbance at this wavelength (consistent with common microplate reader-based detection methods for peroxidase), researchers can accurately quantify S-POD activity in soil samples, providing objective data support for related scientific investigations.
Targeted for Soil Samples: Specifically optimized for the detection of S-POD activity in soil samples, addressing the unique matrix characteristics of soil (e.g., potential interference from organic matter or minerals) to ensure accurate and reliable results.
Sufficient Detection Capacity: Provides 120 test reactions (120T) and can process up to 50 soil samples (50S), making it suitable for both small-scale experiments and medium-throughput sample analysis (e.g., batch testing of soil samples from different experimental plots or sampling points).
Compatible with Standard Laboratory Equipment: Designed for use with a microplate reader—a common piece of equipment in most research laboratories—eliminating the need for specialized or expensive detection instruments and reducing barriers to use.
Comprehensive Testing Accessories: Includes a 96-well plate as a standard testing accessory, saving researchers the time and effort of preparing additional consumables and ensuring consistency in experimental setup.
Clear Storage Guidelines: Provides specific label-based storage instructions to maintain the stability and activity of reagents, extending the usable life of the kit and ensuring reproducibility of results across different experimental batches.
Time-Efficient Operation: Enables the completion of detection for 50 soil samples within approximately 2 hours, significantly shortening the experimental cycle compared to more complex or time-consuming traditional detection methods (e.g., manual colorimetry with repeated reagent preparation).
Simplified Experimental Workflow: Requires only the preparation of a single additional reagent (ether) as specified, minimizing the complexity of pre-experimental reagent preparation and reducing the risk of operational errors caused by excessive reagent handling.
High Reproducibility: The kit’s standardized reagents, pre-included 96-well plate, and microplate reader-compatible design ensure consistent reaction conditions across samples and experiments, leading to highly reproducible results—critical for scientific research data validation.
User-Friendly for Diverse Operators: The straightforward experimental steps and clear instructions (implied by standard assay kit design) make it accessible to both experienced researchers and laboratory technicians with basic molecular biology or soil analysis skills, reducing the learning curve for use.
Relevant to Multiple Research Fields: Supports a wide range of research applications, including soil ecology, environmental pollution monitoring, agricultural soil management, and soil remediation studies, meeting the diverse needs of researchers in different sub-disciplines.
For research use only, not for clinical use.
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