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| Product Name | Six-Channel 96-Well Real-Time PCR System |
| Catalog No. | SM-HMM-0089 |
| Description | A PC-controlled 96-well real-time PCR platform with six fluorescence channels, side excitation and scanning, precise thermal control and integrated analysis software. |
| Intended Use | Absolute and relative quantification; genotyping; positive/negative discrimination; melting-curve analysis. |
| Principle / Technology | A precision thermal cycler combines side-excitation fluorescence optics with real-time signal acquisition and software-based quantitative analysis. |
| Detection Method | Six-channel real-time fluorescence detection with side excitation and side scanning. |
| Sample Type | PCR reactions in compatible 0.2 mL transparent 96-well plates, eight-tube strips or single tubes. |
| Performance Range / Specifications | Reaction volume 10–120 µL; repeatability CV <1%; linear range 1–10¹⁰ copies; excitation 475–670 nm; emission 515–680 nm; maximum/average ramp rates 5/3.5°C/s; temperature uniformity ±0.25°C; temperature accuracy ±0.15°C; six independently controlled thermal zones; gradient span 1–32°C with selectable 30–100°C range; heated lid 25–110°C, default 104°C; dimensions 600 × 390 × 320 mm; weight 23 kg; power 220 V AC, 50/60 Hz, 1,500 VA. |
| Sensitivity / LOD | Low-background optical design supports sensitive fluorescence detection. |
| Specificity | Multichannel optical separation supports dye-specific signal acquisition. |
| Reaction Conditions / Protocol | Create or import a PCR program in the Windows-based software, load a compatible transparent 96-well format and monitor amplification and analysis in real time. |
| Components / Formulation | Main real-time PCR instrument, power cord, USB data cable, USB storage device and replacement fuses. |
| Storage Conditions | Operate indoors at 10–30°C and 20–80% relative humidity, at an altitude below 2,000 m. |
| Package Specifications | 1 instrument with standard accessories. |
| Product Form | Benchtop 96-well real-time PCR instrument. |
| Quality Control | Temperature accuracy, temperature uniformity, fluorescence repeatability, linearity and optical performance are controlled during instrument manufacture. |
| Key Features | Six fluorescence channels; precise six-zone thermal control; low background; user-friendly PC software; closed reaction format; report export and run-history management. |
| Compatibility | F1: FAM/SYBR Green/EvaGreen/LC Green/fluorescein; F2: VIC/HEX/TET/Cy3/JOE/Alexa 555; F3: ROX/Cy3.5/Texas Red; F4: Cy5/LC Red 640; F5: Cy5.5/LC Red 705; customizable F6. Compatible with 0.2 mL transparent non-skirted or semi-skirted 96-well plates, transparent eight-tube strips and transparent single tubes. |
| Application Notes / Precautions | Requires connection to a PC. Use transparent 0.2 mL reaction vessels; white plates are not supported because fluorescence is collected from the side. |
For research use only, not for clinical use.
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