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| Product Name | Rat TGF beta 1 ELISA Kit |
| Catalog No. | EC-0023 |
| Description | Rat TGF beta 1 ELISA kit is a kit for the in vitro detection of TGF beta 1 protein using enzyme-linked adsorption reaction. |
| Species Characteristics | Rat |
| Cross-reaction | No cross reactivity with other rat cytokines. |
| Specificity | Recognizes both natural and recombinant rat TGF-b1. |
| Application | It can be used for immediate in vitro detection of TGF beta 1. |
| Storage | Store at 4°C. |
| Transportation Condition | Transportation at room temperature. |
Transforming Growth Factor-beta 1 (TGF-beta 1) is a key multifunctional cytokine belonging to the TGF-beta superfamily, which plays pivotal roles in regulating a wide range of biological processes across multiple physiological and pathological contexts—making the detection of rat TGF-beta 1 particularly critical for preclinical research.
In rodent models, TGF-beta 1 is extensively studied due to its involvement in:
Tissue Homeostasis and Repair: It mediates extracellular matrix (ECM) synthesis and remodeling, a core process in wound healing, fibrosis, and tissue regeneration. For example, in rat models of liver or kidney fibrosis, abnormal upregulation of TGF-beta 1 is a well-documented driver of collagen deposition and organ dysfunction, making its quantification essential for evaluating anti-fibrotic therapies.
Immune Regulation: As a potent immunosuppressive cytokine, TGF-beta 1 modulates the differentiation and activity of T cells (e.g., promoting regulatory T cell formation), B cells, and macrophages. In rat studies of autoimmune diseases (such as experimental autoimmune encephalomyelitis, EAE) or transplantation, measuring TGF-beta 1 levels helps assess immune response modulation and treatment efficacy.
Developmental Biology: From embryonic development to postnatal growth, TGF-beta 1 regulates cell proliferation, differentiation, and apoptosis. Rat models investigating skeletal development (e.g., bone formation) or embryonic organogenesis rely on accurate TGF-beta 1 detection to unravel developmental pathways.
Pathological Mechanisms: In rat models of cancer (e.g., breast cancer, glioma), TGF-beta 1 exhibits dual roles—suppressing tumor growth in early stages but promoting metastasis and epithelial-mesenchymal transition (EMT) in advanced stages. Quantifying its expression is vital for understanding tumor progression and testing targeted therapies.
Traditional methods for detecting TGF-beta 1 (such as Western blotting or immunohistochemistry) often require large sample volumes, complex sample processing, or lack quantitative precision. The Rat TGF beta 1 ELISA Kit addresses these limitations by leveraging the enzyme-linked immunosorbent assay (ELISA) technique—offering a fast, sensitive, and high-throughput solution tailored for rat samples. This kit is designed to support researchers in academia, pharmaceutical companies, and contract research organizations (CROs) working on rodent-based studies, enabling reliable quantification of TGF-beta 1 in various sample types (e.g., serum, plasma, cell culture supernatants, tissue homogenates) to advance preclinical research outcomes.
Species-Specific Targeting: Exclusively optimized for rat TGF-beta 1 detection, with no cross-reactivity to other rat cytokines (e.g., TGF-beta 2, TGF-beta 3, IL-6, TNF-alpha). This ensures that results accurately reflect only rat TGF-beta 1 levels, eliminating interference from homologous molecules and reducing false-positive/negative readings.
Broad Sample Compatibility: Validated for use with multiple common rat sample matrices, including serum, plasma (EDTA/heparin-anticoagulated), cell culture supernatants, and tissue homogenates. This flexibility allows researchers to analyze TGF-beta 1 across different experimental setups without needing separate detection kits.
Dual Recognition of Natural and Recombinant TGF-beta 1: The kit’s antibodies specifically bind to both endogenously expressed (natural) rat TGF-beta 1 and recombinant rat TGF-beta 1. This feature is valuable for researchers using recombinant proteins as standards or positive controls, ensuring consistency between in vitro and in vivo sample analyses.
Convenient Storage and Transportation: Requires only refrigerated storage (4°C) rather than ultra-low temperature (-20°C or -80°C), reducing the risk of reagent degradation during long-term storage. Additionally, it can be transported at room temperature, simplifying logistics and lowering shipping costs for international or remote research teams.
Ready-to-Use Format: Comes with pre-optimized reagents (including capture antibodies, detection antibodies, enzyme conjugates, substrates, and standards) and detailed protocols. This minimizes hands-on preparation time, reduces experimental variability from reagent mixing, and makes the kit accessible to both experienced and novice researchers.
High Sensitivity and Precision: The kit has a low limit of detection (LOD) typically ≤ 10 pg/mL (validated against industry benchmarks for rat TGF-beta 1 ELISA kits) and a coefficient of variation (CV) < 10% for intra-assay and < 15% for inter-assay. This high sensitivity enables detection of low-abundance TGF-beta 1 in samples (e.g., early-stage tumor microenvironment supernatants), while low CV ensures reproducible results across experiments and labs.
Time-Efficient Workflow: The entire assay can be completed in approximately 3.5–4 hours (from sample preparation to result readout), compared to 6–8 hours for traditional Western blotting or 24+ hours for immunohistochemistry. This high-throughput capability allows researchers to process up to 96 samples (or 192 samples with the 2 x 96 T format) in a single run, accelerating data collection for large-scale studies.
Cost-Effective for Routine Use: Priced competitively relative to other commercial rat TGF-beta 1 ELISA kits, with no compromise on quality. The 1 x 96 T and 2 x 96 T format options allow labs to choose the size that matches their sample volume needs (e.g., small-scale pilot studies vs. large preclinical trials), reducing reagent waste and overall research costs.
Dedicated Technical Support: Includes access to a team of experienced technical specialists who can provide guidance on sample preparation, assay troubleshooting, and data analysis. This support is particularly valuable for researchers encountering unexpected challenges (e.g., high background signal, sample matrix interference), ensuring minimal downtime and reliable results.
For research use only, not for clinical use.
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