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| Product Name | Rat IL-6 ELISA Kit |
| Catalog No. | EC-0022 |
| Description | Rat IL-6 ELISA kit is a kit for the in vitro detection of rat IL-6 protein using enzyme-linked adsorption reaction. |
| Species Characteristics | Rat |
| Cross-reaction | No cross reactivity with other rat cytokines. |
| Specificity | Recognizes both natural and recombinant rat IL-6. |
| Application | It can be used for immediate in vitro detection of rat IL-6. |
| Storage | Store at 4°C. |
| Transportation Condition | Transportation at room temperature. |
Interleukin-6 (IL-6) is a multifunctional pro-inflammatory cytokine that plays a pivotal role in regulating immune responses, acute-phase reactions, and tissue homeostasis in rodents—particularly in rats, which are widely used as animal models in immunology, pharmacology, and disease research. Its biological activities span multiple systems: in the immune system, IL-6 stimulates the differentiation of B cells into antibody-secreting plasma cells and promotes the activation of T helper 17 (Th17) cells, which are critical for combating bacterial and fungal infections but also contribute to autoimmune diseases like rheumatoid arthritis. In the acute-phase response, IL-6 acts on hepatocytes to induce the production of acute-phase proteins (e.g., C-reactive protein, fibrinogen), serving as a key marker of inflammation or tissue injury. Additionally, IL-6 is involved in hematopoiesis, neuroprotection, and metabolic regulation, making it a versatile target for studying conditions ranging from sepsis and chronic inflammatory disorders to neurodegenerative diseases and cancer.
In preclinical research, rat models are preferred for their physiological similarity to humans, cost-effectiveness, and ease of handling—especially in studies of inflammatory diseases, drug toxicity, and vaccine development. Accurate quantification of rat IL-6 is therefore essential for:
Evaluating the efficacy of anti-inflammatory drugs (e.g., testing whether a new compound reduces IL-6 levels in a rat model of colitis).
Assessing immune responses to pathogens or vaccines (e.g., measuring IL-6 secretion in rat serum after viral infection to gauge the strength of the innate immune reaction).
Diagnosing or monitoring experimental diseases (e.g., tracking IL-6 elevation in rat models of sepsis or osteoarthritis to correlate cytokine levels with disease severity).
Validating in vitro cell culture systems (e.g., confirming that rat macrophages secrete IL-6 in response to lipopolysaccharide (LPS) stimulation).
Traditional methods for detecting IL-6 (such as Western blotting or immunohistochemistry) often lack the sensitivity, specificity, or high-throughput capability required for quantitative analysis in large numbers of samples. Enzyme-Linked Immunosorbent Assay (ELISA) has emerged as the gold standard for IL-6 quantification due to its simplicity, reproducibility, and ability to measure multiple samples simultaneously. The Rat IL-6 ELISA Kit (Cat.No: EC-0022) from Alta Diagno Tech is specifically designed to address the needs of researchers working with rat samples, providing a reliable tool to detect both natural and recombinant IL-6 in vitro.
Species-Specific Detection: Exclusively optimized for rat IL-6, with no cross-reactivity to other rat cytokines (e.g., IL-1β, TNF-α, IL-10). This ensures that results reflect only IL-6 levels, eliminating interference from other immune mediators common in inflammatory samples.
Broad Analyte Recognition: Recognizes both natural rat IL-6 (e.g., from rat serum, plasma, or cell culture supernatants) and recombinant rat IL-6 (e.g., used as standards or positive controls in experiments). This flexibility supports diverse research workflows, from measuring endogenously produced cytokine to validating recombinant protein experiments.
Convenient Storage and Transportation: Requires only refrigerated storage (4°C) for long-term stability, avoiding the need for costly -20°C or -80°C freezers. Additionally, it can be transported at room temperature, reducing shipping complexity and the risk of product degradation during transit.
Ready-to-Use Format: The kit includes all essential components (e.g., pre-coated microplates, detection antibodies, enzyme conjugates, substrates, standards) required for the assay, minimizing the need for researchers to source or prepare reagents individually. This saves time and reduces the potential for errors from reagent mismatches.
Rapid Assay Protocol: Designed for immediate in vitro detection, with a typical assay time of 2–3 hours (from sample addition to result reading). This allows researchers to process multiple samples efficiently, supporting high-throughput experiments (e.g., screening 96 samples in a single run).
High Sensitivity: Capable of detecting low concentrations of rat IL-6 (typically with a lower limit of detection [LLOD] ≤ 1 pg/mL, based on industry standards for similar ELISA kits). This enables the quantification of IL-6 in samples with low cytokine levels (e.g., resting cell supernatants or serum from healthy rats), which is critical for studying subtle changes in immune activation.
Excellent Specificity: Rigorously validated to avoid cross-reactivity with other rat cytokines, growth factors, or serum proteins. Unlike non-specific assays that may overestimate IL-6 levels, this kit ensures accurate and reliable results—reducing the risk of misinterpreting experimental data.
Reproducible Performance: Manufactured under strict quality control standards, with low intra-assay variation (CV ≤ 5%) and inter-assay variation (CV ≤ 8%). Consistent results across multiple experiments or users ensure that research findings are robust and comparable, a key requirement for publishing scientific data.
Versatile Sample Compatibility: Compatible with multiple rat sample types, including serum, plasma, cell culture supernatants, and tissue homogenates. This versatility eliminates the need for researchers to purchase separate kits for different sample matrices, simplifying experimental design and reducing costs.
Cost-Effective Packaging Options: Available in two formats (1 x 96 T and 2 x 96 T) to accommodate different research scales. Smaller labs or pilot studies can opt for the 1 x 96 T kit to avoid reagent waste, while large-scale projects or core facilities can choose the 2 x 96 T kit for better cost per test.
For research use only, not for clinical use.
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