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| Product Name | Rat IL-2 ELISA Kit |
| Catalog No. | EC-0028 |
| Description | Rat IL-2 ELISA kit is a kit for the in vitro detection of rat IL-2 protein using enzyme-linked adsorption reaction. |
| Species Characteristics | Rat |
| Specificity | Recognizes both natural and recombinant rat IL-2. |
| Application | It can be used for immediate in vitro detection of rat IL-2. |
| Storage | Store at 4°C. |
| Transportation Condition | Transportation at room temperature. |
Interleukin-2 (IL-2) is a critical cytokine predominantly secreted by activated CD4+ T helper 1 (Th1) cells, with minor contributions from activated CD8+ T cells and natural killer (NK) cells. It serves as a central regulator of the adaptive immune response, playing pivotal roles in T cell proliferation, differentiation, and survival—making it a key target for research in immunology, oncology, and autoimmune disease.
Traditional methods for detecting IL-2, such as Western blotting or flow cytometry, often face limitations that hinder efficient research workflows:
Western blotting requires large sample volumes (typically 20–50 μL per sample) and involves time-consuming steps like protein extraction, gel electrophoresis, and membrane transfer, which can take 4–6 hours to complete. It also only provides semi-quantitative results, making it challenging to compare IL-2 expression levels across multiple samples.
Flow cytometry demands specialized equipment (e.g., a flow cytometer) and trained personnel to operate, increasing experimental costs. It focuses on detecting IL-2 in individual cells rather than measuring total IL-2 concentrations in bulk samples (e.g., serum, cell culture supernatants), limiting its applicability for certain research scenarios.
In contrast, the Enzyme-Linked Immunosorbent Assay (ELISA) technology has emerged as the gold standard for IL-2 detection in research laboratories worldwide. ELISA combines high specificity (via antibody-antigen binding) with excellent sensitivity and quantitative capabilities, addressing the gaps of traditional methods. Our Rat IL-2 ELISA Kit (Cat.No: EC-0028) is engineered based on this robust ELISA platform, specifically optimized for detecting rat IL-2—a species widely used in preclinical studies. Rats are preferred in immunological research due to their well-characterized immune system, which closely mimics human immune responses in models of autoimmune diseases (e.g., rheumatoid arthritis, multiple sclerosis), transplant rejection, and anti-tumor immunity. By enabling accurate, rapid, and cost-effective detection of rat IL-2, this kit supports researchers in unlocking insights into immune activation, therapeutic efficacy, and disease pathogenesis.
Broad Target Recognition: Specifically identifies both natural rat IL-2 (isolated from rat serum, plasma, or cell culture supernatants) and recombinant rat IL-2 (expressed in bacterial, yeast, or mammalian expression systems), ensuring compatibility with diverse experimental setups.
Versatile Sample Compatibility: Validated for use with multiple common rat sample types, including cell culture supernatants (e.g., from activated T cell cultures), serum (collected via standard venipuncture), and plasma (EDTA or heparin-anticoagulated), eliminating the need for specialized sample preparation kits.
Stable Storage & Transportation: Requires only refrigerated storage at 4°C (no frozen storage needed) and can be transported at room temperature, reducing logistics complexity and the risk of product degradation during shipping—ideal for international or remote laboratory orders.
User-Friendly Protocol: Comes with a detailed, step-by-step instruction manual that includes pre-calibrated reagent volumes, incubation times, and troubleshooting tips. The entire assay can be completed in 3–4 hours (including sample preparation), minimizing hands-on time for researchers.
Quantitative Precision: Generates precise, concentration-based results (not just qualitative or semi-quantitative readouts) using a standard curve, allowing researchers to quantify IL-2 levels in pg/mL or ng/mL—essential for statistical analysis of experimental data.
Superior Sensitivity: Has a lower limit of detection (LOD) of ≤ 10 pg/mL for rat IL-2, outperforming many competing kits (which often have LODs of 20–50 pg/mL). This high sensitivity enables detection of low-abundance IL-2 in samples with minimal cytokine secretion (e.g., resting T cell cultures or early-stage immune responses).
Exceptional Specificity: Uses highly purified, monoclonal antibodies against rat IL-2 that exhibit no cross-reactivity with other rat cytokines (e.g., IL-4, IL-6, IFN-γ) or IL-2 from other species (e.g., mouse, human, rabbit). This eliminates false-positive results and ensures accurate IL-2 quantification.
Cost-Effective Format: Available in two practical sizes (1 x 96 T and 2 x 96 T) to match different experimental scales. The 1 x 96 T format is ideal for small-scale projects (e.g., pilot studies with 20–30 samples), while the 2 x 96 T format reduces per-assay costs for large-scale experiments (e.g., high-throughput drug screening with 100+ samples).
Consistent Reproducibility: Undergoes rigorous quality control (QC) testing, with each batch validated for inter-assay precision (coefficient of variation, CV ≤ 10%) and intra-assay precision (CV ≤ 8%). This ensures that results are consistent across different experiments, operators, or time points—critical for reliable research outcomes.
No Specialized Equipment Required: Works with a standard microplate reader (capable of measuring absorbance at 450 nm), a common tool in most research laboratories. This eliminates the need for investing in expensive, specialized equipment (e.g., flow cytometers or Luminex systems).
For research use only, not for clinical use.
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