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| Product Name | Rapid Colorimetric Mycoplasma Detection Kit |
| Catalog No. | BT-QCY-0095 |
| Description | A rapid isothermal colorimetric assay for routine detection of mycoplasma contamination in cell-culture supernatants. The closed-tube workflow does not require PCR instrumentation or post-amplification electrophoresis. |
| Intended Use | Routine screening of adherent and suspension cell cultures for mycoplasma contamination. |
| Principle / Technology | Isothermal amplification generates a visual color change that indicates the presence of mycoplasma nucleic acid. |
| Detection Method | Visual colorimetric endpoint after constant-temperature incubation. |
| Sample Type | Cell-culture supernatant; serum can also be tested directly. |
| Performance Range / Specifications | Uses 1 µL sample; detects up to 28 mycoplasma species, including eight commonly associated with cell-culture contamination. |
| Sensitivity / LOD | At least 500 CFU in 1 µL culture supernatant, equivalent to approximately 5 × 10^5 CFU/mL. |
| Specificity | Broad detection of common cell-culture mycoplasma with tolerance to culture-supernatant inhibitors. |
| Reaction Conditions / Protocol | Add 1 µL supernatant to the reaction system, incubate at 60°C for 1 hour and interpret the color at the specified endpoint. |
| Storage Conditions | Store at -30 to -15°C. |
| Package Specifications | 20 reactions; 50 reactions. |
| Product Form | Colorimetric isothermal detection kit. |
| Key Features | One-hour result; direct supernatant testing; no PCR instrument; no electrophoresis; closed-tube detection; broad media compatibility. |
| Shipping Conditions | Ship at 0°C or below. |
| Compatibility | CHO, Vero, hybridoma, Sf9, HEK293 and other adherent or suspension culture systems. |
| Application Notes / Precautions | Do not open reaction tubes after amplification. Interpret at 60 minutes because prolonged incubation can produce unreliable color development. |
For research use only, not for clinical use.
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