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| Product Name | Quick T4 DNA Ligation Mix |
| Catalog No. | RESK-0075 |
| Description | This product is a ready-to-use 2x premix for molecular cloning. It is simple to operate and completes ligation of insert fragments into vectors in 5 minutes at 25 °C, saving experimental time. The mix is suitable not only for sticky-end ligation but also for blunt-end ligation and TA ligation. In addition, its reaction performance remains stable after repeated freeze-thaw cycles. |
| Intended Use | Rapid ligation of DNA insert fragments into vectors for molecular cloning, including sticky-end ligation, blunt-end ligation, and TA cloning. For research use only. |
| Principle / Technology | T4 DNA ligase catalyzes the formation of phosphodiester bonds between adjacent 5'-phosphate and 3'-hydroxyl termini in duplex DNA, joining insert and vector fragments. |
| Performance Range / Specifications | Fast: ligation completed in 5 minutes. Highly efficient: high cloning positive rate. Stable reaction performance after repeated freeze-thaw cycles. |
| Reaction Conditions / Protocol | Ligate the insert fragment and vector at 25 °C for 5 min using the ready-to-use 2x premix. |
| Components / Formulation | Ready-to-use 2x ligation premix |
| Storage Conditions | Store at -25 to -15 °C. |
| Shelf Life | 18 months |
| Package Specifications | 20 T 100 T |
| Product Form | Liquid 2x premix |
| Expiration Date / Stability | Stable for 18 months at -25 to -15 °C; reaction performance remains stable after repeated freeze-thaw cycles. |
| Compatibility | Compatible with sticky-end ligation, blunt-end ligation, and TA ligation. |
For research use only, not for clinical use.
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