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| Product Name | One-Step Colorimetric Mycoplasma Detection Kit |
| Catalog No. | BT-QCY-0094 |
| Description | A one-tube isothermal amplification assay for rapid visual detection of mycoplasma contamination in adherent and suspension cell cultures. Enzyme and buffer components are premixed for direct sample addition. |
| Intended Use | Routine monitoring of mycoplasma contamination in cultured cells. |
| Principle / Technology | Isothermal nucleic acid amplification with a colorimetric endpoint and a dUTP/UDG carryover-contamination control system. |
| Detection Method | Visual colorimetric readout; negative reactions remain red and positive reactions turn yellow. |
| Sample Type | Cell-culture supernatant from adherent or suspension cultures. |
| Performance Range / Specifications | Detects more than 40 mycoplasma species, including eight common cell-culture contaminants; uses 1 µL sample per reaction. |
| Sensitivity / LOD | Reported detection of mycoplasma-positive plasmid down to 100 copies/µL. |
| Specificity | Designed for broad mycoplasma detection with compatibility across common culture media and serum types. |
| Reaction Conditions / Protocol | Add 1 µL cell-culture supernatant to the premixed reaction and incubate at 65°C for 1 hour before visual interpretation. |
| Components / Formulation | One-tube enzyme and buffer premix with dUTP/UDG anti-contamination chemistry. |
| Storage Conditions | Store at -30 to -15°C. |
| Package Specifications | 25 reactions; 50 reactions. |
| Product Form | Colorimetric isothermal amplification kit. |
| Key Features | One-tube workflow; direct sample addition; one-hour result; visual endpoint; broad species coverage; carryover-contamination control. |
| Shipping Conditions | Ship at 0°C or below. |
| Compatibility | Adherent and suspension cell cultures; multiple culture-media and serum formulations. |
| Application Notes / Precautions | Interpret results at the specified one-hour endpoint and keep reaction tubes closed after amplification to reduce aerosol contamination. |
For research use only, not for clinical use.
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