- Home
- IVD
- By Technology Types
- By Diseases Types
- By Product Types
- Research
- Resource
- Distributors
- Company
| Product Name | Murine MCP-1 ELISA Kit |
| Catalog No. | EC-0020 |
| Description | Murine MCP-1 ELISA kit is a kit for the in vitro detection of murine MCP-1 protein using enzyme-linked adsorption reaction. |
| Species Characteristics | Murine |
| Specificity | Recognizes both natural and recombinant murine MCP-1. |
| Application | It can be used for immediate in vitro detection of murine MCP-1. |
| Storage | Store at 4°C. |
| Transportation Condition | Transportation at room temperature. |
Monocyte Chemoattractant Protein-1 (MCP-1, also known as CCL2) is a key member of the CC chemokine family, playing an indispensable role in regulating immune responses, inflammation processes, and disease progression—making the detection of murine MCP-1 critical for preclinical research in immunology, oncology, neuroscience, and cardiovascular disease.
The Biological Significance of Murine MCP-1
Immune Cell Recruitment: As a potent chemoattractant, murine MCP-1 specifically binds to its receptor CCR2 (expressed on monocytes, macrophages, and activated T cells), guiding these immune cells to sites of inflammation or tissue damage. This process is essential for initiating innate immune responses and resolving infections.
Inflammation Regulation: Abnormal expression of murine MCP-1 is closely linked to chronic inflammatory diseases. For example, in murine models of rheumatoid arthritis, elevated MCP-1 levels in joint synovial fluid promote macrophage infiltration and cartilage destruction; in inflammatory bowel disease (IBD) models, MCP-1 mediates intestinal mucosal inflammation by recruiting pro-inflammatory immune cells.
Tumor Microenvironment Modulation: In oncology research, murine MCP-1 contributes to tumor progression and metastasis. It recruits tumor-associated macrophages (TAMs)—a major component of the tumor microenvironment—that secrete growth factors (e.g., VEGF) and matrix metalloproteinases (MMPs) to support tumor angiogenesis and invasion. Studies in murine breast cancer and melanoma models have confirmed that targeting MCP-1/CCR2 signaling reduces TAM infiltration and inhibits tumor growth.
Neurological and Cardiovascular Research: In murine models of stroke, MCP-1 is upregulated in ischemic brain tissue, where it recruits microglia to clear necrotic cells but may also exacerbate neuroinflammation. In atherosclerosis models, MCP-1 drives monocyte migration into arterial walls, a key step in plaque formation.
The Need for Reliable Murine MCP-1 Detection Tools
Preclinical studies using murine models rely heavily on accurate quantification of MCP-1 to:
Validate the efficacy of anti-inflammatory or anti-tumor drugs (e.g., testing if a novel CCR2 inhibitor reduces MCP-1-induced immune cell recruitment).
Elucidate disease mechanisms (e.g., measuring MCP-1 levels in knockout mice to confirm its role in a specific pathway).
Monitor treatment responses (e.g., tracking changes in MCP-1 expression in serum or tissue homogenates during therapy).
Traditional detection methods such as Western blotting or immunofluorescence lack quantitative precision, while non-specific immunoassays may cross-react with other chemokines (e.g., MCP-2, MCP-3). The Murine MCP-1 ELISA Kit (Cat.No: EC-0020) addresses these limitations by providing a highly specific, sensitive, and reproducible in vitro detection solution—aligning with the rigorous demands of modern preclinical research.
Broad Sample Compatibility: Designed to detect murine MCP-1 in multiple common sample types used in preclinical research, including murine serum, plasma (EDTA- or heparin-anticoagulated), cell culture supernatants (e.g., from murine macrophages or tumor cell lines), and tissue homogenates (e.g., from murine liver, brain, or tumor tissues). This flexibility eliminates the need for multiple kits for different sample sources, simplifying experimental workflows.
Dual Specificity for Natural and Recombinant Targets: Recognizes both endogenously expressed natural murine MCP-1 (e.g., from murine tissue or serum) and recombinant murine MCP-1 (e.g., used as a positive control in cell stimulation assays). This ensures consistency across experiments involving both native biological samples and recombinant proteins.
Stable Storage and Transportation: Can be stored at 4°C for extended periods (per kit instructions) without loss of performance, reducing the need for frequent reordering. Additionally, it supports room-temperature transportation—eliminating the cost and logistical complexity of cold-chain shipping, which is critical for researchers in regions with limited refrigerated transport infrastructure.
User-Friendly Protocol: The kit includes pre-optimized reagents (e.g., pre-coated microplates, ready-to-use detection antibodies, and stabilized HRP-conjugated streptavidin) and a step-by-step manual. Even researchers with limited ELISA experience can complete the assay in ~3.5 hours (including incubation and washing steps), minimizing experimental errors and saving time.
High Sensitivity for Low-Abundance Samples: With a minimum detectable limit (MDL) as low as 16.2 pg/mL (validated against industry benchmarks for murine MCP-1 ELISA kits), the kit can accurately quantify MCP-1 in samples with low expression levels—such as murine serum (where basal MCP-1 concentrations are typically <50 pg/mL) or early-stage inflammatory tissue homogenates. This outperforms less sensitive kits (MDL >50 pg/mL) that may miss biologically relevant changes in MCP-1 levels.
Exceptional Specificity with No Cross-Reactivity: Rigorous testing confirms no cross-reactivity with other murine chemokines (e.g., MCP-2, MCP-3, MCP-4, MCP-5), human MCP-1, or unrelated proteins (e.g., MARC). This eliminates false-positive results, a common issue with low-quality ELISA kits, ensuring that detected signals directly reflect murine MCP-1 concentrations.
Superior Reproducibility for Reliable Data: The kit exhibits minimal intra-assay and inter-assay variability, with coefficient of variation (CV) values <10% for both. Intra-assay CV (variability within a single microplate) ensures consistency across samples tested in parallel, while inter-assay CV (variability across multiple plates) guarantees reliable results over long-term experiments—critical for publishing reproducible research data.
Cost-Effective for Scaled Experiments: Available in two formats (1×96 T and 2×96 T), the kit caters to both small-scale pilot studies (e.g., 1×96 T for initial drug screening) and large-scale experiments (e.g., 2×96 T for testing multiple time points or dose groups). The 2×96 T format offers a lower cost per test compared to purchasing two 1×96 T kits, reducing overall research expenses.
For research use only, not for clinical use.
|
There is no product in your cart. |
Copyright © 2026 Alta DiagenoTech. All rights reserved.
