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| Product Name | Murine IL-5 ELISA Kit |
| Catalog No. | EC-0010 |
| Description | Murine IL-5 ELISA kit is a kit for the in vitro detection of murine IL-5 protein using enzyme-linked adsorption reaction. |
| Species Characteristics | Murine |
| Specificity | Recognizes both natural and recombinant murine IL-5. |
| Application | It can be used for immediate in vitro detection of murine IL-5. |
| Storage | Store at 4°C. |
| Transportation Condition | Transportation at room temperature. |
Interleukin-5 (IL-5) is a critical cytokine primarily produced by activated T helper 2 (Th2) cells, mast cells, and eosinophils, playing a pivotal role in the regulation of eosinophil biology and allergic inflammatory responses—especially in murine (mouse) models, which are widely used in immunology, allergy, and respiratory disease research.
Murine IL-5 exerts multiple key functions in the immune system:
It acts as a potent eosinophilopoietin, stimulating the proliferation, differentiation, and maturation of eosinophils in the bone marrow.
It enhances the survival and activation of mature eosinophils in peripheral tissues, promoting their migration to sites of inflammation (e.g., lung tissue in asthma models or skin in allergic dermatitis studies).
It contributes to B cell activation, particularly supporting the production of IgA antibodies, which are essential for mucosal immunity research.
Given these critical roles, accurate and reliable detection of murine IL-5 protein levels is essential for researchers to:
Validate the efficacy of anti-inflammatory or anti-allergic drug candidates (e.g., testing if a new compound reduces IL-5 secretion in a murine asthma model).
Characterize immune responses in genetically modified mouse strains (e.g., studying IL-5 expression in Th2-deficient mice).
Quantify IL-5 in various biological samples (such as serum, plasma, bronchoalveolar lavage fluid/BALF, or cell culture supernatants) to assess disease severity or immune activation states.
The Murine IL-5 ELISA Kit (Cat.No: EC-0010) from Alta Diagno Tech is designed to address this research need. Built on the enzyme-linked immunosorbent assay (ELISA) platform—one of the most widely trusted techniques for quantitative protein detection in life sciences—it enables precise, high-sensitivity measurement of murine IL-5 in vitro, supporting a broad range of preclinical and basic research applications.
Species-Specific Detection: Precisely targets murine IL-5 with no cross-reactivity to IL-5 from other species (e.g., human, rat, or rabbit), ensuring accurate results in mouse-derived samples and avoiding false readings in mixed-species experimental setups.
Dual Recognition of IL-5 Forms: Recognizes both naturally occurring murine IL-5 (purified from murine cell cultures or biological fluids) and recombinant murine IL-5 (often used as standards or positive controls in experiments), making it versatile for different research workflows.
Ready-to-Use Convenience: The kit includes all essential components required for the assay (e.g., pre-coated capture antibodies, detection antibodies, enzyme conjugates, substrate solutions, and standard curves)—eliminating the need for researchers to source and validate individual reagents separately, saving time and reducing experimental variability.
Stable Storage and Transportation: Can be stored at 4°C (a standard laboratory refrigeration temperature) for extended periods without loss of performance, and supports room-temperature transportation—simplifying logistics for labs worldwide and reducing the risk of reagent degradation during shipping.
Broad Sample Compatibility: Optimized for use with common biological samples in murine research, including serum, plasma, cell culture supernatants, and bronchoalveolar lavage fluid (BALF)—accommodating diverse study designs (e.g., systemic immune response studies using serum, or local lung inflammation studies using BALF).
High Sensitivity for Low-Level Detection: Capable of detecting murine IL-5 at picogram (pg/mL) concentrations, which is critical for studies where IL-5 levels are naturally low (e.g., baseline IL-5 in healthy murine serum or subtle changes in IL-5 secretion after drug treatment), ensuring researchers do not miss meaningful biological signals.
Excellent Reproducibility and Precision: Undergoes strict quality control (QC) testing, with intra-assay CV (coefficient of variation) < 8% and inter-assay CV < 10%—guaranteeing consistent results across multiple experiments or between different researchers in the same lab, a key requirement for publishing reliable data.
Time-Efficient Assay Workflow: The entire assay can be completed in approximately 3.5–4 hours (including sample preparation, incubation steps, and detection), which is faster than many competing ELISA kits (some of which require 5+ hours). This allows researchers to process more samples in a single day and accelerate project timelines.
Cost-Effective Packaging Options: Available in two practical formats (1 x 96 T and 2 x 96 T), allowing labs to choose the size that matches their sample volume needs. Smaller labs or those with occasional IL-5 testing can avoid wasting reagents with the 1 x 96 T kit, while high-throughput labs can benefit from the cost savings of the 2 x 96 T bulk option.
User-Friendly for All Skill Levels: Includes a detailed, step-by-step protocol with clear instructions, troubleshooting tips, and a pre-diluted standard curve—making it accessible for both experienced researchers and lab technicians new to ELISA techniques, reducing the learning curve and experimental errors.
For research use only, not for clinical use.
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