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Cat.No: HMA-1604 Datasheet Instruction for Use
Specification Quantities
Price $457.05–761.75
| Product Name | Intrinsic factor pAb |
| Catalog No. | HMA-1604 |
| Description | A polyclonal antibody against intrinsic factor (IF), the glycoprotein essential for vitamin B12 absorption in the ileum. Ideal for research on pernicious anemia, autoimmune gastritis, and B12 metabolism studies. |
| Test Procedure | WB: 1:500 - 1:1000 ELISA: Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements. Western blot analysis of lysates from Mouse stomach, using GIF Rabbit pAb (A24479) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3s |
| Host | Rabbit |
| Isotype | IgG |
| Molecular Weight | Observed MW: 55kDa Calculated MW: 42kDa/45kDa |
| Purity | Antigen-specific affinity purification |
| Reconstituted Form | PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3 |
| Immunogen | Recombinant protein (or fragment).This information is considered to be commercially sensitive. |
| Applications | WB, ELISA |
| Storage and Transportation | Store at -20°C. Avoid freeze / thaw cycles. |
For research use only, not for clinical use.
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