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| Product Name | Influenza Virus AM2 RT-PCR Kit |
| Catalog No. | VT-QCY-0023 |
| Application | The test kit can be used for rapid detection of influenza virus AM2. |
| Test Methods | PCR |
| Features | Fast and easy: One minute operation time, fifteen minutes room temperature reaction. Versatile: No specific restriction enzyme cleavage site or recombination site is required, PCR or enzyme cleavage products can be cloned. High efficiency and high throughput: Simple transformation, positive rate up to 90% or more. |
| Storage | Store at -20°C and avoid repeated freezing and thawing. |
Influenza viruses pose a significant threat to both animal and human health, with subtype-specific variations (such as AM2) often linked to outbreaks in research contexts—including studies on avian, swine, and equine influenza transmission dynamics, as well as viral evolution. The Influenza Virus AM2 RT-PCR Kit is developed to address the critical need for reliable, rapid detection of the AM2 subtype in scientific research, filling a gap in pathogen analysis workflows for laboratories focused on virology, epizootiology, and public health-related research (non-clinical).
Research Significance of AM2 Subtype: The AM2 gene, closely associated with the matrix (M) and hemagglutinin (HA) gene families in influenza A viruses, plays a key role in viral structure stability and host cell interaction. Studying AM2-positive influenza strains is essential for understanding subtype-specific pathogenicity, cross-species transmission potential, and the development of experimental countermeasures—all of which rely on accurate, early detection of the virus in research samples.
Limitations of Traditional Detection Methods: Conventional techniques for influenza detection, such as viral culture or immunological assays, often require 24–72 hours for results, have lower sensitivity (failing to detect <100 viral copies), and lack subtype specificity. These drawbacks hinder timely progress in research projects focused on influenza epidemiology, viral mutation tracking, and experimental vaccine testing.
Role of This RT-PCR Kit in Research: Designed exclusively for research use (not clinical diagnostics), this kit leverages reverse transcription-polymerase chain reaction (RT-PCR) technology to target the AM2 gene, enabling researchers to rapidly identify the subtype in diverse samples—including avian oropharyngeal swabs, swine nasal secretions, and experimental cell culture supernatants. Its high specificity and efficiency support high-throughput research workflows, such as large-scale sample screening for influenza surveillance studies or validation of viral propagation in laboratory models.
Ultra-Rapid Operation & Reaction: Requires only 1 minute of hands-on preparation time (e.g., adding sample to pre-optimized reagents) and completes the entire RT-PCR reaction in 15 minutes at room temperature—eliminating the need for time-consuming temperature cycling delays, which significantly accelerates research timelines compared to traditional 1–2 hour PCR protocols.
No Restriction on Sample or Product Use: Does not require samples to contain specific restriction enzyme cleavage sites or recombination sites, providing flexibility for research samples with varied viral genetic backgrounds. Additionally, PCR or enzyme cleavage products generated by the kit can be directly used for cloning experiments—supporting follow-up research such as viral gene sequencing or recombinant protein expression.
High Sensitivity for Low Viral Loads: Capable of detecting as few as <100 copies of the AM2 viral target per sample, ensuring researchers can identify low-abundance viral presence in early-stage infections or dilute experimental samples (e.g., post-dilution cell culture supernatants), which avoids false-negative results that could compromise research data.
Broad Compatibility with Research Samples: Validated for use with a wide range of research samples, including avian fecal samples, swine bronchoalveolar lavage fluid (BALF), equine nasal swabs, and mammalian cell culture lysates. This versatility eliminates the need for multiple kits for different sample types, simplifying laboratory inventory management.
Stable Storage & Minimal Degradation Risk: Stores at -20°C with clear instructions to avoid repeated freezing and thawing—ensuring reagent stability for long-term use (typically 6–12 months when stored correctly). The lyophilized format of key components (where applicable) further reduces degradation risks during transport and storage, maintaining consistent performance across research batches.
High Positive Rate Ensures Research Reliability: Delivers a positive detection rate of 90% or higher for AM2-positive samples, minimizing false-negative results that could lead to incorrect research conclusions (e.g., misclassifying a viral strain as non-AM2 subtype). This high reliability is critical for studies requiring rigorous data validation, such as viral prevalence surveys or experimental infection models.
Cost-Effective for High-Throughput Research: Each kit provides 50 test reactions, with a low cost per data point—ideal for laboratories conducting large-scale research projects (e.g., screening hundreds of field samples for influenza surveillance). The optimized reagent mix also reduces the need for additional consumables (e.g., separate reverse transcriptase or primers), lowering overall research costs.
Simplified Protocol Reduces Human Error: Comes with a ready-to-use reagent system (pre-mixed primers, probes, and reverse transcriptase) that requires no manual reagent preparation or optimization. This simplifies operation for researchers with varying PCR experience levels and minimizes errors from pipetting or reagent mixing—ensuring consistent results across different users or experimental batches.
Built-In Controls Support Data Validation: Includes internal extraction controls (to verify nucleic acid extraction quality) and positive/negative controls (to confirm assay performance), which help researchers troubleshoot experimental issues (e.g., failed extraction vs. true negative samples) and meet the rigorous data quality standards required for scientific publications or research grants.
Broad Dynamic Detection Range: Offers a dynamic range of >6 logs, meaning it can accurately detect the AM2 subtype across a wide range of viral concentrations (from <100 copies to 10^6 copies per sample). This flexibility allows researchers to quantify viral load changes in time-course experiments (e.g., tracking viral replication in cell cultures over 72 hours) without diluting samples multiple times.
For research use only, not for clinical use.
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