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| Product Name | Human IL-27 ELISA Kit |
| Catalog No. | EC-0073 |
| Description | Human IL-27 ELISA kit is a kit for the in vitro detection of human IL-27 protein using enzyme-linked adsorption reaction. |
| Species Characteristics | Human |
| Cross-reaction | No cross reactivity with other human cytokines. |
| Specificity | Recognizes both natural and recombinant human IL-27. |
| Application | It can be used for immediate in vitro detection of human IL-27. |
| Storage | Store at 4°C. |
| Transportation Condition | Transportation at room temperature. |
Interleukin-27 (IL-27), a key member of the interleukin cytokine family, plays a pivotal role in regulating immune responses and maintaining immune homeostasis. Composed of two distinct subunits, IL-27p28 and EBI3, it exerts diverse biological effects that make its accurate detection critical for both basic research and clinical investigation.
In the context of immune regulation, IL-27 is widely recognized for its ability to modulate T cell function. It participates in the differentiation and activation of T helper 1 (Th1) cells, working in coordination with other cytokines like IL-12 and IL-23 to fine-tune anti-microbial immune responses. Beyond its role in anti-microbial immunity, IL-27 also acts as a negative regulator of excessive inflammation, helping to prevent tissue damage caused by overactive immune reactions—this dual role makes it a focal point in studies related to autoimmune diseases, chronic inflammation, and infectious diseases.
The demand for reliable IL-27 detection tools has grown rapidly in recent years, driven by the expanding scope of research in areas such as neuroinflammatory diseases, tumor biology, and infectious diseases. Traditional detection methods often faced challenges like low sensitivity, cross-reactivity with other cytokines, or complex operational workflows, which limited their applicability in detecting IL-27 in low-concentration samples (e.g., cerebrospinal fluid, tears) or high-throughput experimental settings.
To address these limitations, Alta Diagno Tech has developed the Human IL-27 ELISA Kit (Cat.No: EC-0073), leveraging the double-antibody sandwich ELISA technology—a gold standard in commercial cytokine detection. This technology is favored for its high specificity in identifying target analytes in complex biological samples, along with advantages like simple operation, cost-effectiveness, and stable signal output. The Human IL-27 ELISA Kit is specifically designed to detect human IL-27 protein in vitro, with no cross-reactivity with other human cytokines and the ability to recognize both natural and recombinant forms of human IL-27. It effectively meets the needs of researchers in fields such as immunology, molecular biology, and clinical medicine for accurate, efficient, and reliable IL-27 detection.
Human-Specific Detection with High Specificity: The kit is exclusively optimized for human IL-27 detection, ensuring no cross-reactivity with other human cytokines. It can specifically recognize both natural and recombinant human IL-27 protein, eliminating false-positive results caused by non-target molecule interference.
Convenient Storage and Transportation: The kit can be stored at 4°C, a standard laboratory storage condition that avoids the need for expensive cryogenic storage equipment. Additionally, it supports transportation at room temperature, reducing logistics costs and the risk of product damage during transportation.
Versatile Application for Immediate Detection: It is suitable for immediate in vitro detection of human IL-27, compatible with common biological sample types used in IL-27 research (e.g., plasma, serum, cell culture supernatants). This versatility allows researchers to apply the kit across different experimental scenarios without the need for additional sample preprocessing adjustments.
Stable Experimental Performance: Built on the double-antibody sandwich ELISA platform, the kit delivers consistent and stable signal output. The experimental system supports repeated plate reading, and reliable results can still be obtained even if plate reading is delayed (e.g., reading after overnight storage or 48 hours), providing flexibility for experimental scheduling.
Simplified Operational Workflow: Adopting the classic double-antibody sandwich ELISA technology, the kit features a straightforward operation process that does not require complex steps such as plate coating or multiple washing cycles. Researchers only need to follow standard ELISA protocols for sample addition and incubation, reducing the learning curve for new users and minimizing human errors caused by complicated operations.
Cost-Effective Solution for Research: Compared to high-sensitivity but high-cost detection technologies (e.g., Single Molecular Array), the kit offers a cost-effective alternative while maintaining sufficient detection performance for most routine IL-27 research needs. It is particularly suitable for laboratories with limited budgets or large-scale experimental projects that require multiple detections.
Low Sample Consumption Potential: Aligned with the sample-saving characteristics of commercial ELISA kits, the kit is designed to minimize sample usage. Although specific sample volume requirements are tailored to experimental needs, it typically only requires a small amount of sample (consistent with the 1-6 μL to 20 μL range of common cytokine ELISA kits) to complete detection, making it ideal for precious samples with limited availability (e.g., cerebrospinal fluid, rare clinical specimens).
Broad Compatibility with Experimental Scales: The kit is available in multiple formats, including 1 x 48 T, 1 x 96 T, and 2 x 96 T. This allows researchers to select the appropriate package size based on the number of samples (e.g., 48 T for small-scale preliminary experiments, 96 T or 2 x 96 T for high-throughput experiments), avoiding waste of reagents and optimizing experimental costs.
For research use only, not for clinical use.
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