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Cat.No: DBX-QCY-1679 Datasheet Instruction for Use
Specification Quantities
Price $1086-1810
| Product Name | Human FDP ELISA Kit |
| Catalog No. | DBX-QCY-1679 |
| Description | This assay is based on the sandwich enzyme-linked immunosorbent assay (ELISA) technique. Human Fibrinogen Degradation Product (FDP) present in the sample is specifically bound by an immobilized capture antibody and subsequently recognized by a biotinylated detection antibody, forming a sandwich immune complex. This complex is further associated with an avidin horseradish peroxidase (HRP) conjugate, enabling enzymatic signal amplification. Upon reaction with a chromogenic substrate, the HRP enzyme catalyzes the development of a colored product. The resulting signal intensity, measured spectrophotometrically at 450 ± 2 nm, is directly proportional to the concentration of Human FDP in the sample. Quantitative determination is achieved by comparison with a standard curve generated from known concentrations. |
| Kit Components | Micro ELISA plate Reference standard Concentrated biotinylated detection Ab (100×) Concentrated HRP conjugate (100×) Reference standard & sample diluent Biotinylated detection Ab diluent HRP conjugate diluent Concentrated wash buffer (25×) Substrate reagent Stop solution Plate sealing film Manual |
| Materials Required But Not Provided | Microplate reader capable of measuring absorbance at 450 nm High-precision pipettes, EP tubes, and disposable pipette tips Incubator capable of maintaining 37 °C Deionized or distilled water Absorbent paper Loading slot (Suction Tank) |
| Sample Type | Serum, plasma, cell culture supernatant |
| Sensitivity | 0.94 ng/mL |
| Detection Range | 1.56-100 ng/mL |
| Assay Method | Sandwich ELISA |
| Test Procedure | Assign wells for diluted standards, blanks, and samples. Add 100 μL of each standard, blank, or sample to the designated wells. Analyze all samples and standards in duplicate. Determine sample dilution factors based on preliminary experiments or technical support recommendations. Cover the plate with the supplied sealer and incubate for 90 minutes at 37 °C. Remove liquid from each well without washing. Add 100 μL of biotinylated detection Ab working solution to each well. Cover with a fresh sealer and incubate for 1 hour at 37 °C. Decant the solution and add 350 μL of Wash Buffer to each well. Soak for 1 minute, then aspirate or decant and blot dry with clean absorbent paper. Note: A microplate washer may be used. Do not allow wells to dry. Add 100 μL of HRP conjugate working solution to each well. Cover the plate with a new sealer. Incubate for 30 min at 37 °C. Repeat the wash procedure, described in Step 3, five times. Add 90 μL of substrate reagent to each well. Cover and incubate for approximately 15 min at 37°C. Protect the plate from light. Note: Reaction time may be adjusted based on observed color development but should not exceed 30 minutes. Switch on the microplate reader for 15 min prior to OD measurement. Add 50 μL of stop solution to each well in the same order as the substrate addition. Measure the optical density (OD value) at 450 ± 2 nm using a microplate reader immediately after adding stop solution . |
| Interpretation of Results | Data Processing. Calculate the mean optical density (OD) of duplicate readings for each standard and sample. Subtract the mean OD of the zero standard (blank) from all easurements. Standard Curve. Plot a four-parameter logistic (4PL) curve with standard concentrations on the x-axis (log scale optional) and OD values on the y-axis. Sample Concentration. Determine the concentration of each sample from the standard curve. If the sample OD exceeds the upper limit of the standard curve, repeat the assay using an appropriate dilution. The final concentration is calculated using the following equation:Final concentration = Measured concentration × Dilution factor |
| Storage and Transportation | Store at 2–8°C for 6 months. Ship refrigerated. |
| Precautions | This kit is intended for research purposes and is not for diagnostic or therapeutic use. Always wear lab coats, safety goggles, and disposable gloves when handling reagents or biological samples. Follow local and national biosafety regulations, especially when working with blood, plasma, or other bodily fluids. Protect light-sensitive reagents such as Concentrated HRP Conjugate (100×) and Substrate Reagent from exposure to light. Ensure all reagent caps are tightly closed to prevent evaporation and microbial contamination. Do not reuse reconstituted standards, working solutions of detection antibody, or HRP conjugate. Stock solutions must be stored according to specified conditions. Do not mix reagents from different kit lots or sources. Pipetting and Cross-Contamination Prevention Use fresh tips for each standard, sample, and reagent addition. Use separate reservoirs for each reagent to prevent cross contamination. Do not use the kit beyond the expiration date indicated on the label. |
For research use only, not for clinical use.
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