| Product Name | Human Erythrocyte RH(D/CE) Blood Group Genotyping Kit (PCR-SBT) |
| Catalog No. | BTRTK-HMM-0015 |
| Description | The main reasons for the generation of common RhD difficult blood groups are pathologic disturbances and subtypes. Prediction of RhD and RhCE blood groups can provide a reference for genetic research on RHD and RHCE blood groups. |
| Applications | Prefabricated 24-well amplification dry plate per person and universal forward/reverse sequencing primer sets for all amplification wells; RHD and RHCE blood group genes are amplified and analyzed at the same time, and auxiliary reading holes are added to determine the source of sequencing results for all RHD defects and RHCE*C holes; sequencing region includes 1-10 exons and peripheral intronic sequences; and the target sequences can be detected by selecting the amplification well positions. |
| Sample Type | Whole blood ≥1 mL (sodium citrate or EDTA anticoagulation); DNA ≥24 μL (concentration 20-40 ng/μL, A260/A280 value 1.6-2.0); Amniotic fluid: ≥4 mL (more than 16 weeks), the above three kinds of specimens were stored at 2-8°C for 2 weeks, and ≤-20°C for 1 year. Oral mucosa swabs (2, respectively, with brushing force to collect oral mucosa cells from both cheeks, stored at room temperature for 1 week). |
| Detection Methods | PCR-SSP/Sanger Sequencing |
| Estimated Measurement Time | 3 h |
| Shelf Life | 18 months |
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