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Cat.No: BT-QCY-0101 Datasheet Instruction for Use
Specification Quantities
Price $2060.28–3433.80
| Product Name | Enterococcus hirae Probe Realtime PCR Kit (Without Internal Control) |
| Catalog No. | BT-QCY-0101 |
| Description | A probe-based real-time PCR kit for specific detection of Enterococcus hirae DNA without an internal control. Designed for veterinary microbiology, animal pathogen screening, and environmental monitoring research. |
| Features | Ready-to-use; users only need to provide a DNA template sample. The primers and probes have been optimized to ensure high analytical sensitivity, capable of detecting as few as 100 copies per reaction. A positive control is provided to help distinguish false-negative results. High specificity; primers are designed based on highly conserved regions of Enterococcus faecalis DNA and do not cross-react with DNA from other organisms. Suitable for both qualitative and quantitative detection. The linear range for quantitative detection is at least 5 orders of magnitude. This product is sufficient for 50 probe-based qPCR reactions in a 20 μL system. This product is for research use only. |
| Kit Components | 2× ProbeqPCR Master Mix: 0.5 mL Fluorescent PCR Template Dilution Buffer: 1 mL Ultrapure water: 1 mL Enterococcus faecalis qPCR Primer-Probe Powder: 50 reactions Enterococcus faecalis qPCR Positive Control (1E7 copies/μL): 50 μL User manual: 1 copy |
| Materials Required But Not Provided | Sample DNA, and nucleic acid purification kit |
| Assay Method | PCR |
| Sample Preparation | If there are N samples, it is best to prepare N + 2 extractions: one additional sample serves as the PC (positive control for sample preparation), and another as the NC (negative control for sample preparation). To prepare the PC, use 10 μL of the 4th dilution solution obtained in the previous step and add an appropriate amount of water to bring the total volume to match the initial sample volume required for each sample preparation. Use water as the NC. Purify the sample DNA using a method of your choice. This kit is compatible with most commercially available DNA extraction kits; alternatively, you may purchase our company’s extraction-free nucleic acid release reagent. |
| Test Procedure | If only a single replicate quantitative analysis is performed, label N + 9 PCR tubes, of which N + 2 are for the N + 2 samples obtained, 1 is for the PCR negative control (using water as a template), and 6 are for the standard curve. If only a single replicate qualitative analysis is performed, label N + 4 PCR tubes: N + 2 for the N + 2 samples obtained in the previous step, 1 for the PCR negative control (using water as a template), and 1 for the PCR positive control. Finally, perform the PCR reaction using the samples. |
| Results | If the positive control for sample preparation or the PCR positive control (including the standard curve sample) yields a negative result, the entire experiment is invalid. There is no need to analyze the data; instead, repeat the sample preparation and PCR amplification, or contact the manufacturer. If the negative control for sample preparation or the PCR negative control yields a positive result, indicating environmental contamination, the entire experiment is invalid. There is no need to analyze the data; contact the manufacturer. m. If both the negative control and the positive control are normal, the experiment is valid and may proceed to subsequent analysis. If this kit is used for quantitative detection, plot a standard curve with the log value of the positive control concentration on the x-axis and the Ct value on the y-axis. Then, use the Ct values of the test samples to calculate the log values of the sample DNA concentrations from the standard curve, and subsequently determine the actual concentrations. If this kit is used for qualitative testing (to determine only positive or negative results), the Ct value of the negative control must be unreadable or greater than or equal to 40. The positive control must exhibit a logarithmic increase in fluorescence, a typical amplification curve, and a Ct value less than 40. For test samples, if the Ct value is not detected or is greater than or equal to 40, the result is negative; if it is less than 40, the result is positive. |
| Storage and Transportation | Transport at low temperatures; store at -20°C. Shelf life: 12 months. |
For research use only, not for clinical use.
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