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| Product Name | Cell Cycle and Apoptosis Assay Kit, Flow Cytometry |
| Catalog No. | REKI-0021 |
| Description | Flow cytometry kit that measures cell cycle distribution and apoptosis in a single staining workflow. The red fluorescent signal is acquired on the 488 nm channel, so DNA content and sub-diploid apoptotic populations can be read from the same sample. Only 70 percent ethanol and PBS need to be prepared in the laboratory, which keeps the protocol short between fixation and acquisition. Store the kit at -20 °C away from light; the components keep for 12 months. |
| Intended Use | Combined flow cytometric analysis of cell cycle distribution and apoptosis |
| Principle / Technology | A DNA-binding fluorescent dye reports DNA content, and apoptotic sub-populations appear as distinct peaks |
| Specificity | 50 T; red fluorescence detected on the 488 nm channel; ethanol and PBS prepared by the user; 12 month shelf life |
| Reaction Conditions / Protocol | Fix the cells in 70 percent ethanol, stain with the supplied reagent, then acquire red fluorescence on a flow cytometer at 488 nm |
| Storage Conditions | -20 °C, protected from light |
| Package Specifications | 50 T |
| Key Features | Combined cycle and apoptosis readout; single 488 nm channel; 12 month shelf life |
For research use only, not for clinical use.
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