| Product Name | Activated Partial Thromboplastin Time (APTT) Test Kit |
| Catalog No. | CFTK-HMM-0108 |
| Application | Activated Partial Thromboplastin Time (APTT) Test Kit (Ellagic Acid Coagulation Method) is used for the determination of activated clotting time of human and animal blood, which is one of the more sensitive and commonly used screening experiments to reflect the endogenous coagulation system. |
| Detection Principle | The time it takes for venous blood to clot completely after it is separated from the body is the clotting time, which is a screening test reflecting the activity of each coagulation factor in the endogenous coagulation system. The activated partial thromboplastin time (APTT) is a screening test that reflects the activity of the endogenous coagulation system. The APTT is calculated using the time required for plasma to clot in the absence of platelets by activating factor XII with ellagic acid at 37°C, replacing the platelets with the partially active coagulant to provide the catalytic surface for coagulation, and converting the fibrinogen to an insoluble kilo-dimensional protein with the participation of Ca2+. |
| Self-contained Reagents / Instruments / Consumables | Centrifuges, timers, water baths |
| Procedures | 1. Preparation of plasma to be tested: Take fresh venous blood to be tested, mix with sodium citrate anticoagulant (109 mM) at 9:1 and mix gently. Centrifuge at 3000 rpm/min for 10 min, collect the upper layer of liquid (platelet-poor plasma) and transfer it to a plastic test tube or centrifuge tube to prevent platelet activation. A normal control plasma should be set up at the same time. 2. Equilibrate the APTT ellagic acid solution to room temperature and mix upside down. Take the plasma to be tested (including normal control plasma) and 0.1 mL each of APTT ellagic acid solution and mix well. 3. placed in a 37 ℃ water bath for 5 min, during which time gently mixed several times. 4. add the incubation to 37 ℃ CaCl2 (25 mM) 0. 1 mL, immediately timing, placed in the water bath and constantly shaking, about 30 s when removed, observe the emergence of fibrin filaments of time, repeat 2 times to take the average value. |
| Note | 1. Plasma can be collected by sodium citrate anticoagulant tube, not EDTA, heparin, oxalate, etc. as anticoagulant, samples should be tested promptly after obtaining, generally should not be more than 2 h, and the incubation time at 37 ℃ should not be less than 3 min. 2. Separate plasma should be centrifuged at 3000 r/min for 10 min, be sure to remove platelets. 3. The APTT ellagic acid solution should not be preheated for more than 15 min. 4. The experimental material must not contain detergent, otherwise the results of the assay can be falsely prolonged. 5. Measurement temperature 36.5-38.5 ℃, too low or too high can cause false prolongation. 6. Operation of sufficient light, thrombin endpoint calculation to appear turbid initial coagulation shall prevail. 7. APTT ellagic acid solution may appear yellow precipitate after long-term storage, can be shaken evenly before use. 8. Oral contraceptives, estrogen, pregnancy, coumarins, asparaginase, etc. may affect APTT results. |
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