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| Product Name | Rat Crh (Corticotropin Releasing Hormone) ELISA Kit |
| Catalog No. | HTK-YJL-0015 |
| Synonyms | Corticoliberin ELISA Kit, Corticotropin-releasing Factor ELISA Kit, CRF ELISA Kit, Corticotropin-releasing Hormone ELISA Kit, CRH ELISA Kit |
| Species | Rat |
| UniProt ID | P01143 |
| Sample Type | Serum, plasma, cell culture supernatant, cell or tissue lysate, other liquid samples |
| Detection Method | Competitive ELISA, Coated with Antigen |
| Detection Wavelength | OD450 |
| Reaction Duration | 2 hours |
| Range | 0.313-20 ng/ml |
| Sensitivity | 0.188 ng/ml |
| Storage | 2-8°C (Sealed). Don't cryopreserve. |
| Specificity | Specifically binds with Crh, no obvious cross reaction with other analogues. |
| Intended Use | For research use only. |
Corticotropin Releasing Hormone (CRH), also known as corticotropin-releasing factor (CRF) or corticoliberin, is a key regulatory peptide in the hypothalamic-pituitary-adrenal (HPA) axis— the body’s primary system for responding to stress. Produced mainly by the paraventricular nucleus (PVN) of the hypothalamus, CRH acts on the anterior pituitary gland to stimulate the secretion of adrenocorticotropic hormone (ACTH). ACTH then travels to the adrenal cortex, triggering the release of glucocorticoids (e.g., corticosterone in rats), which help the body adapt to stress by regulating metabolism, immune function, and mood.
Beyond its core role in stress regulation, CRH is involved in multiple physiological and pathological processes. In neuroscience research, it is studied for its links to anxiety, depression, and neuroinflammation, as abnormal CRH levels are often associated with dysregulation of the HPA axis in these conditions. In endocrinology, CRH measurements are critical for investigating disorders related to HPA axis dysfunction, such as Cushing’s syndrome or adrenal insufficiency.
For researchers working with rat models (a widely used species in preclinical stress, neuroscience, and endocrinology studies), accurate quantification of CRH in biological samples is essential to understand its role in experimental contexts. Our Rat CRH ELISA Kit is designed to meet this need, enabling precise detection of CRH in common rat sample types, supporting advancements in stress biology, neuropharmacology, and endocrine research.
High Specificity: Specifically binds to rat CRH with no significant cross-reactivity with CRH analogues or other related peptides, ensuring that test results reflect true CRH concentrations in samples.
Broad Sample Compatibility: Compatible with multiple common rat sample types, including serum, plasma, cell culture supernatant, cell lysates, and tissue lysates, eliminating the need for specialized sample preparation or restricted sample sources.
Rapid Assay Time: Completes the entire detection process in just 2 hours, significantly reducing waiting time for researchers and enabling efficient batch processing of samples.
Clear Detection Wavelength: Uses a standard 450 nm detection wavelength, which is compatible with most common microplate readers in research laboratories, avoiding the need for specialized equipment.
Reliable Storage Conditions: Requires only 2-8°C refrigerated storage (no cryopreservation needed) and maintains stability within the shelf life, simplifying inventory management and reducing the risk of reagent degradation from repeated freeze-thaw cycles.
Well-Defined Detection Range & Sensitivity: Offers a detection range of 0.313-20 ng/ml and a sensitivity of 0.188 ng/ml, covering the typical physiological concentration range of CRH in rat samples and enabling detection of low-level CRH signals.
Precise Quantitative Results: Based on competitive ELISA technology with pre-coated CRH antigen, the kit ensures that the optical density (OD) values measured at 450 nm have a stable inverse relationship with sample CRH concentrations. This allows for accurate quantification via standard curve fitting, supporting reliable data analysis for research projects.
Consistent Performance: Follows rigorous manufacturing and quality control standards, minimizing intra-assay and inter-assay variability (reference to industry common performance: typical intra-assay CV < 10% and inter-assay CV < 10% for similar kits). This consistency ensures that results are reproducible across different experiments or operators.
User-Friendly Protocol: The assay workflow is straightforward, with clear steps for reagent preparation, sample addition, incubation, washing, and detection. This reduces the learning curve for new users and lowers the risk of operational errors, making it suitable for both experienced and novice laboratory personnel.
Cost-Effective Formats: Available in 48T and 96T formats, allowing researchers to choose the appropriate size based on their sample volume needs (e.g., small-scale pilot studies or large-scale batch experiments). This flexibility helps optimize research budgets by avoiding unnecessary reagent waste.
Research-Focused Design: Tailored exclusively for rat CRH detection—aligning with the widespread use of rat models in stress, neuroscience, and endocrinology research. It directly addresses the specific needs of researchers studying HPA axis function, stress-related disorders, or CRH-mediated biological processes.
For research use only, not for clinical use.
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