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| Product Name | NADH Oxidase (NOX) Activity Assay Kit, 110T/50S, Microplate reader |
| Catalog No. | EATK-YJL-0027 |
| Testing Equipment | Microplate reader |
| Number of Detectable Samples | 50 Samples |
| Testing Accessories | 96-well plate |
| Estimated Measurement Time | 4 h (50 Samples) |
| Storage | Store according to label instructions |
| Reagents to be Prepared | None |
NADH oxidase (NOX, EC 1.6.99.3) is a homotetrameric flavoenzyme that is widely distributed in animals, plants, microorganisms and cultured cells, playing a pivotal role in multiple biological processes across different organisms. Its core biological function is to directly oxidize NADH to NAD⁺ in the presence of oxygen, a reaction that is crucial for maintaining the intracellular balance of NADH/NAD⁺, which is essential for the normal operation of glycolysis and other key metabolic pathways.
The enzyme has attracted significant attention in academic and research fields due to its close associations with various physiological and pathological mechanisms. It is closely linked to immune responses, as its activity can influence the body's ability to regulate immune-related reactions. Moreover, NOX is recognized as a well - known cancer - related biomarker; studies have shown that its activity is significantly higher in cancer patients compared to healthy individuals, and inhibiting NOX activity has been found to reduce cancer cell growth and induce cancer cell apoptosis. In addition, it contributes to preventing the accumulation of harmful reactive oxygen species by participating in oxygen reduction reactions, thus protecting cells from oxidative damage.
The development of NOX activity assay kits is driven by the growing demand for accurate detection of this enzyme in scientific research. The colorimetric detection principle adopted by this kit is widely applied in research. It couples the oxidation of NADH by NOX with the reduction of 2,6 - dichlorophenol indophenol (DCPIP). The blue DCPIP is reduced to colorless DCPIP during the reaction, and researchers can calculate the NOX activity by measuring the reduction rate of blue DCPIP at 600nm using a microplate reader. This detection method provides a reliable technical means for in - depth studies on NOX - related metabolic pathways, disease mechanisms and drug development.
It adopts the colorimetric method with an optimal detection wavelength of 600nm, which is compatible with common microplate readers (590 - 610nm is also applicable), enabling convenient and rapid detection in ordinary laboratory environments.
It has a wide applicability to sample types, which can be used for detecting NOX activity in various samples such as animal and plant tissues, cultured cells and microbial samples, meeting the diverse research needs of different fields.
The kit has a clear detection range of 0.38 - 22.09 U/L and a sensitivity of 0.38 U/L, which can accurately detect both low and medium to high levels of NOX activity in samples, ensuring the reliability of detection data for different research scenarios.
The assay process is time - efficient, with the whole detection process achievable within a short time (about 15 - 30 minutes), which helps to improve the efficiency of batch sample detection in research experiments.
The kit components are fully equipped and pre - prepared in a reasonable way. It includes necessary reagents such as lysis buffers and chromogens, simplifying the pre - experimental preparation steps and reducing the operational complexity for researchers.
It has excellent detection precision. The intra - assay coefficient of variation (CV) is less than 4%, and the inter - assay CV is less than 7.1%, which ensures that the detection results are stable and repeatable, avoiding large data deviations caused by experimental operations or reagent differences.
It has a high recovery rate, with an average recovery rate of 102%. This indicates that the kit has little interference on the detection process, and can accurately reflect the actual NOX activity in the samples, laying a solid foundation for reliable research conclusions.
The storage conditions are manageable. The kit can be stored at - 20°C in the dark for up to 12 months, and individual components can be stored separately according to the label prompts, which is convenient for long - term storage in laboratories without frequent reagent replacement.
The sample volume required for detection is small, only 20 μL for tissue or cell homogenate samples. This advantage not only saves precious sample resources, especially for rare samples in research, but also reduces the experimental cost indirectly.
The result calculation method is clear and straightforward. Corresponding calculation formulas are provided for different sample types and statistical standards (such as protein concentration, sample fresh weight), which is easy for researchers to operate and reduces errors in result calculation.
For research use only, not for clinical use.
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