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| Product Name | Murine TGF beta 1 ELISA Kit |
| Catalog No. | EC-0012 |
| Description | Murine TGF beta 1 ELISA kit is a kit for the in vitro detection of murine TGF beta 1 protein using enzyme-linked adsorption reaction. |
| Species Characteristics | Murine |
| Cross-reaction | No cross reactivity with other murine cytokines. |
| Specificity | Recognizes both natural and recombinant murine TGF-b1. |
| Application | It can be used for immediate in vitro detection of murine TGF beta 1. |
| Storage | Store at 4°C. |
| Transportation Condition | Transportation at room temperature. |
Transforming Growth Factor-beta 1 (TGF-beta 1) is a pivotal member of the TGF-beta superfamily, a group of multifunctional polypeptides that regulate a wide spectrum of cellular processes across species. For murine (mouse) models—one of the most widely used experimental systems in biomedical research—TGF-beta 1 plays an indispensable role in both physiological homeostasis and pathological progression, making its accurate detection critical for advancing scientific discoveries.
Key Biological Roles of Murine TGF-beta 1
Cellular Proliferation and Differentiation: Murine TGF-beta 1 acts as a dual regulator, inhibiting the growth of epithelial, endothelial, and hematopoietic cells while promoting the proliferation and differentiation of mesenchymal cells (e.g., fibroblasts, chondrocytes). This balance is essential for tissue development, such as embryonic organ formation and postnatal tissue repair.
Extracellular Matrix (ECM) Remodeling: It stimulates the synthesis of ECM components (e.g., collagen I, fibronectin) and suppresses ECM-degrading enzymes (e.g., matrix metalloproteinases, MMPs) in murine tissues. This function is particularly relevant in studies on wound healing, fibrosis (e.g., liver, lung, kidney fibrosis in mouse models), and musculoskeletal disorders—such as the role of TGF-beta 1 in murine Achilles tendon microdamage repair (as highlighted in research on exercise-induced tendon injury).
Immune Regulation: In murine immune systems, TGF-beta 1 is a key immunosuppressive cytokine. It inhibits the activation and effector functions of T cells, B cells, and macrophages, and promotes the differentiation of regulatory T cells (Tregs) to maintain immune tolerance. This makes it a critical target in studies on murine autoimmune diseases (e.g., experimental autoimmune encephalomyelitis, EAE), inflammatory disorders, and tumor immunology (where aberrant TGF-beta 1 expression often contributes to immune evasion by murine tumors).
Pathological Relevance in Murine Models: Abnormal expression of murine TGF-beta 1 is linked to numerous diseases studied in mouse models, including cancer (e.g., breast cancer, melanoma metastasis), cardiovascular diseases (e.g., atherosclerosis, cardiac fibrosis), and neurodegenerative diseases (e.g., Alzheimer’s disease-related amyloid-beta clearance). Accurate quantification of murine TGF-beta 1 in biological samples (e.g., serum, plasma, cell culture supernatants, tissue lysates) is therefore essential for understanding disease mechanisms, evaluating therapeutic efficacy, and validating experimental hypotheses.
Need for a Specialized Murine TGF-beta 1 ELISA Kit
Traditional protein detection methods (e.g., Western Blot) often lack the sensitivity and quantitative precision required to measure TGF-beta 1, which is typically present at low concentrations (pg/mL range) in murine biological samples. ELISA kits, particularly those optimized for murine TGF-beta 1, address this gap by leveraging specific antibody-antigen interactions to enable high-sensitivity, high-specificity quantitative detection. The Murine TGF-beta 1 ELISA Kit (Cat.No: EC-0012) is designed to meet the rigorous demands of murine research, providing reliable data to support reproducible scientific outcomes.
Species-Specific Detection: Exclusively optimized for murine TGF-beta 1, with no cross-reactivity with other murine cytokines (e.g., TNF-alpha, IL-6, IL-10). This ensures that detected signals are solely from the target protein, eliminating false positives caused by non-specific binding—critical for distinguishing TGF-beta 1 from other closely related cytokines in complex murine samples.
Broad Target Recognition: Recognizes both natural (endogenously expressed in murine tissues/cells) and recombinant (exogenously introduced in experimental systems) murine TGF-beta 1. This versatility allows researchers to use the kit across diverse applications, from measuring endogenously secreted TGF-beta 1 in murine splenocyte cultures to quantifying recombinant TGF-beta 1 in transfection experiments.
Flexible Sample Compatibility: Validated for use with common murine biological samples, including serum, plasma, and cell culture supernatants. This flexibility eliminates the need for multiple kits to analyze different sample types, streamlining experimental workflows for researchers studying murine physiology or disease.
Convenient Storage and Transportation: Requires only refrigerated storage (4°C) and can be transported at room temperature. This simplifies inventory management for laboratories and reduces the risk of product degradation during shipping, ensuring the kit remains functional upon arrival—even for international customers.
Rapid In Vitro Detection: Enables immediate quantitative detection of murine TGF-beta 1 in vitro, with a user-friendly protocol that minimizes hands-on time. This allows researchers to process multiple samples efficiently, accelerating data acquisition and reducing experimental turnaround time.
High Signal Stability: The kit’s reaction system maintains stable signals over time, supporting repeated readouts if needed (e.g., verifying results or adjusting detection parameters). This stability ensures consistent data across multiple measurements, enhancing the reliability of experimental outcomes.
Superior Sensitivity: Designed to detect murine TGF-beta 1 at the pg/mL range, matching the low physiological concentrations of TGF-beta 1 in murine samples. This sensitivity surpasses many general-purpose ELISA kits, enabling the detection of subtle changes in TGF-beta 1 expression—such as slight upregulation during early-stage murine wound healing or downregulation after therapeutic intervention.
Exceptional Specificity: Utilizes highly specific antibodies that bind exclusively to murine TGF-beta 1, with no cross-reactivity to other murine cytokines or human/rat TGF-beta 1. This specificity is critical for avoiding interference from other proteins in complex samples (e.g., murine tissue lysates with high cytokine diversity), ensuring accurate quantification of the target.
Cost-Effective Format: Available in a 1 x 96 T format, which is optimized for small to medium-scale experiments (e.g., analyzing 40-50 samples in triplicate). This format reduces waste compared to larger kits, making it economical for laboratories with moderate sample throughput while maintaining the ability to generate statistically robust data.
User-Friendly Protocol: No specialized technical expertise is required to operate the kit. The step-by-step protocol includes clear instructions for sample preparation, reagent mixing, incubation, and detection, making it accessible to both experienced researchers and laboratory technicians new to ELISA assays.
Reproducible Results: Manufactured under strict quality control standards, with low intra-assay and inter-assay variability. This ensures that results are consistent across different experiments, different operators, and different batches of the kit—critical for meeting the reproducibility requirements of scientific publishing and regulatory compliance (e.g., for preclinical studies).
Time-Efficient Workflow: The kit’s protocol is optimized to minimize incubation times without compromising detection performance. This allows researchers to complete the entire assay (from sample addition to result readout) in a reasonable timeframe, supporting high-throughput experimental designs (e.g., screening multiple murine treatment groups).
For research use only, not for clinical use.
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