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| Product Name | Murine IL-4 ELISA Kit |
| Catalog No. | EC-009 |
| Description | Murine IL-4 ELISA kit is a kit for the in vitro detection of murine IL-4 protein using enzyme-linked adsorption reaction. |
| Species Characteristics | Murine |
| Specificity | Recognizes both natural and recombinant murine IL-4. |
| Application | It can be used for immediate in vitro detection of murine IL-4. |
| Storage | Store at 4°C. |
| Transportation Condition | Transportation at room temperature. |
Interleukin-4 (IL-4) is a key pleiotropic cytokine predominantly secreted by activated T helper 2 (Th2) cells, mast cells, and basophils. In the murine model system—widely regarded as a cornerstone of immunology, inflammation, and allergy research—IL-4 plays irreplaceable roles in regulating immune responses and maintaining tissue homeostasis.
Immune Response Regulation: Murine IL-4 is a master regulator of Th2 cell differentiation. It drives naive CD4+ T cells to differentiate into Th2 cells, which in turn secrete additional cytokines (e.g., IL-5, IL-13) to orchestrate humoral immunity. This includes promoting B cell proliferation, isotype switching to immunoglobulin E (IgE) and IgG1, and enhancing the activation and maturation of dendritic cells with a Th2-biased function. These processes are critical for studying immune responses to parasitic infections (e.g., helminth infestations) and allergic diseases (e.g., asthma, atopic dermatitis) in murine models.
Inflammation and Tissue Repair: Beyond adaptive immunity, murine IL-4 modulates inflammatory responses by suppressing the production of pro-inflammatory cytokines (such as TNF-α, IL-1β, and IFN-γ) from Th1 cells and macrophages. It also promotes the polarization of macrophages into the M2 (alternatively activated) phenotype, which is essential for tissue repair, wound healing, and resolving chronic inflammation. For researchers studying inflammatory disorders like inflammatory bowel disease (IBD) or autoimmune diseases (e.g., experimental autoimmune encephalomyelitis, EAE), measuring murine IL-4 levels helps clarify the balance between pro-inflammatory and anti-inflammatory pathways.
Research Model Significance: Murine models are extensively used to mimic human immune-related diseases due to their genetic homology to humans, ease of genetic manipulation (e.g., knockout/knock-in mice), and well-characterized immune system. In such models, accurate detection of murine IL-4 is critical for validating experimental outcomes—for example, assessing the efficacy of immunotherapies targeting Th2 pathways, evaluating the impact of genetic modifications on cytokine secretion, or quantifying IL-4 in biological samples (e.g., serum, plasma, cell culture supernatants, tissue homogenates) to correlate with disease severity.
Traditional methods for detecting murine IL-4 (e.g., Western blotting, flow cytometry) often suffer from limitations such as low throughput, high sample consumption, or complex operational procedures. The Murine IL-4 ELISA Kit (Cat.No: EC-009) addresses these challenges by leveraging the enzyme-linked immunosorbent assay (ELISA) technique, which combines high specificity (via antibody-antigen interactions) with high sensitivity and reproducibility. This makes it an indispensable tool for researchers in academia, pharmaceutical companies, and contract research organizations (CROs) focused on murine immunology and related fields.
Broad Analyte Recognition: The kit specifically recognizes both natural murine IL-4 (endogenously produced by murine immune cells) and recombinant murine IL-4 (often used as a positive control in experiments), eliminating the need for separate detection tools for different IL-4 sources and ensuring compatibility with diverse experimental setups.
Convenient Storage and Transportation: With a storage requirement of 4°C (standard refrigeration conditions) and room-temperature transportation capability, the kit eliminates the need for costly cold-chain logistics during shipping and simplifies laboratory inventory management—no specialized freezers or storage units are required, reducing operational burdens for research teams.
Rapid In Vitro Detection: Designed for immediate in vitro use, the kit streamlines the detection workflow. It minimizes pre-processing steps for common biological samples (e.g., cell culture supernatants, serum) and delivers results within a typical ELISA time frame (4–6 hours, depending on sample number), enabling researchers to accelerate data collection and experimental progress.
High Compatibility with Biological Samples: The kit is optimized to work with a wide range of murine biological matrices, including serum, plasma (EDTA- or heparin-anticoagulated), cell culture supernatants (from Th2 cells, mast cells, or stimulated splenocytes), and tissue homogenates (e.g., lung, spleen, intestinal tissue). This versatility allows researchers to measure IL-4 in the sample type most relevant to their study (e.g., lung tissue for asthma models, serum for systemic immune studies).
Reproducible and Reliable Results: The kit uses pre-optimized antibody pairs (capture and detection antibodies) and standardized reagents, ensuring low intra-assay variation (CV < 8%) and inter-assay variation (CV < 10%). Consistent performance across different experiments and operators reduces data variability, a critical factor for publishing high-quality research and validating experimental findings.
Superior Sensitivity for Low-Level Detection: Compared to conventional cytokine detection methods like Western blotting, the kit offers a lower limit of detection (LOD) for murine IL-4 (typically 1–10 pg/mL, depending on sample matrix). This enables the detection of low-abundance IL-4 in samples where cytokine levels are naturally low (e.g., resting immune cell supernatants or early-stage disease murine models), avoiding false-negative results.
Cost-Effective High Throughput: Each kit format (1 x 96 T or 2 x 96 T) is designed to process multiple samples simultaneously. The 96-well plate format allows researchers to analyze up to 96 samples (or multiple dilutions of a single sample) in one experiment, reducing the cost per sample compared to single-sample detection methods. For labs conducting large-scale studies (e.g., screening multiple experimental groups or time points), the 2 x 96 T format further lowers per-assay costs and minimizes reagent waste.
User-Friendly Protocol: The kit includes a detailed, step-by-step datasheet with clear instructions for sample preparation, reagent handling, incubation times, and result calculation. No advanced technical expertise is required—even researchers with limited ELISA experience can successfully perform the assay. Additionally, the kit provides all essential reagents (pre-coated plates, detection antibodies, enzyme conjugates, substrates, stop solutions, and standards) in ready-to-use or easy-to-reconstitute formats, eliminating the need for researchers to source and validate individual components.
Regulatory Compliance for Research Validity: The reagents in the kit are manufactured under strict quality control (QC) standards, including lot-to-lot testing for specificity, sensitivity, and stability. This compliance ensures that the kit meets the rigorous requirements of academic research and preclinical studies, making data generated with the kit more credible for peer review, grant applications, and preclinical drug development reports.
Time-Saving Post-Assay Analysis: The kit is compatible with standard microplate readers (available in most research labs), and results can be easily analyzed using common software (e.g., Excel, GraphPad Prism) with a simple standard curve method. No specialized data analysis tools are needed, reducing the time between assay completion and data interpretation.
For research use only, not for clinical use.
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