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| Product Name | Murine IL-17F ELISA Kit |
| Catalog No. | EC-0017 |
| Description | Murine IL-17F ELISA kit is a kit for the in vitro detection of murine IL-17F protein using enzyme-linked adsorption reaction. |
| Species Characteristics | Murine |
| Specificity | Recognizes both natural and recombinant murine IL-17F. |
| Application | It can be used for immediate in vitro detection of murine IL-17F. |
| Storage | Store at 4°C. |
| Transportation Condition | Transportation at room temperature. |
Interleukin-17F (IL-17F) is a key member of the IL-17 cytokine family, which plays a pivotal role in regulating immune responses, inflammatory processes, and host defense against pathogens—especially in murine (mouse) models, the most widely used animal system in immunology, infectious disease, and inflammatory disorder research.
Murine IL-17F shares structural and functional homology with human IL-17F, making it an essential tool for translating preclinical findings to clinical applications. It is primarily secreted by Th17 cells, a subset of helper T lymphocytes, as well as by innate immune cells such as γδ T cells, natural killer (NK) cells, and mast cells. Once released, murine IL-17F exerts its biological effects by binding to its specific receptor (IL-17RA/IL-17RC complex) on target cells, triggering downstream signaling pathways that promote the production of pro-inflammatory cytokines (e.g., TNF-α, IL-6), chemokines (e.g., CXCL8, CCL2), and antimicrobial peptides.
In research settings, accurate detection of murine IL-17F levels is critical for understanding:
The pathogenesis of inflammatory and autoimmune diseases (e.g., rheumatoid arthritis, psoriasis, inflammatory bowel disease, and multiple sclerosis) in mouse models.
Host immune responses to bacterial, viral, or fungal infections (e.g., murine models of Staphylococcus aureus skin infection or influenza A virus lung infection, where IL-17F contributes to pathogen clearance).
The efficacy of novel therapeutic agents (e.g., anti-IL-17F antibodies, Th17 cell modulators) in preclinical trials, as changes in murine IL-17F expression often correlate with treatment success or failure.
Traditional methods for detecting murine IL-17F (such as Western blotting or flow cytometry) have limitations—they require large sample volumes, involve complex protocols, or lack the sensitivity needed to measure low concentrations of IL-17F in biological fluids (e.g., serum, plasma, bronchoalveolar lavage fluid, or cell culture supernatants). The Murine IL-17F ELISA Kit addresses these gaps by leveraging the enzyme-linked immunosorbent assay (ELISA) technology, a gold standard for quantitative, high-throughput, and user-friendly detection of proteins in vitro.
Broad Specificity for Murine IL-17F Forms: Recognizes both naturally occurring murine IL-17F (endogenously produced by murine immune cells in tissues or biological fluids) and recombinant murine IL-17F (exogenously expressed in cell systems for research use), ensuring compatibility with diverse experimental designs.
Rapid In Vitro Detection: Enables quick quantitative analysis of murine IL-17F—with a standard assay workflow (sample preparation, incubation, washing, enzyme reaction, and signal reading) that can be completed within 3–4 hours, reducing experimental turnaround time compared to labor-intensive methods like Western blotting.
Versatile Sample Compatibility: Validated for use with a wide range of murine biological samples commonly used in research, including serum, plasma (EDTA- or heparin-anticoagulated), cell culture supernatants (e.g., from activated murine Th17 cells or splenocytes), and tissue homogenates (e.g., from murine lung, gut, or joint tissues), eliminating the need for specialized sample pre-treatment.
Stable Storage and Transportation: Requires only refrigerated storage (4°C) for long-term preservation (typically 6–12 months from the date of manufacture, when stored as recommended), and can be transported at room temperature—simplifying logistics and reducing the risk of product degradation during shipping.
User-Friendly Protocol: Comes with a detailed, step-by-step datasheet that includes pre-optimized reagent volumes, incubation times, and washing protocols. No specialized technical expertise is required, making it accessible to both experienced researchers and laboratory technicians new to ELISA assays.
High Sensitivity and Precision: Detects murine IL-17F at concentrations as low as 10 pg/mL (a typical lower limit of detection for high-quality IL-17F ELISA kits, based on market benchmarks) and has a coefficient of variation (CV) of <10% for intra-assay (same plate) and <15% for inter-assay (different plates) measurements—ensuring reliable, reproducible results that meet rigorous scientific publication standards.
Cost-Effective Format: Available in 1×96 T and 2×96 T formats, allowing laboratories to choose the size that matches their sample throughput. The 2×96 T option offers a lower cost per test, making it ideal for high-volume studies (e.g., screening multiple treatment groups in a preclinical trial) without compromising quality.
Minimal Cross-Reactivity: Undergoes rigorous validation to ensure no significant cross-reactivity with other murine cytokines (e.g., IL-17A, IL-17B, IL-6, TNF-α) or human IL-17F—preventing false-positive or false-negative results that could invalidate research data.
Consistent Performance Across Batches: Each lot of the kit is manufactured under strict quality control (QC) standards, with batch-specific validation data (including standard curves, recovery rates, and specificity tests) provided in the datasheet. This ensures that performance remains consistent across different orders, avoiding experimental variability due to product lot differences.
Support for Diverse Research Goals: Whether used for basic research (e.g., studying IL-17F’s role in immune cell differentiation) or translational research (e.g., evaluating drug candidates in murine disease models), the kit provides quantitative data that can be easily analyzed and compared across experiments—accelerating research progress and data interpretation.
For research use only, not for clinical use.
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