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| Product Name | Hydroxyl Free Radical Scavenging Capacity Assay Kit |
| Catalog No. | CMAK-YJL-0113 |
| Size | 96 T (92 samples), 48 T (44 samples) |
| Sample Type | Serum (plasma); tissues |
| Detection Instrument | Microplate reader (500-530 nm, optimal detection wavelength: 510 nm) |
| Research Field | Oxidative stress |
| Accuracy | Inter-assay variation: 4.6% | Intra-assay variation: 4.1% |
| Sample Size | 20 μL |
| Storage | The product can be stored at 2-8°C, protected from light for 12 months. |
| Shelf Life | 12 months |
| Detection Time | 40 min |
| Intended Use | For research use only. |
Hydroxyl free radical (·OH) stands out as one of the most reactive and destructive members of the reactive oxygen species (ROS) family in biological systems. Unlike other ROS such as superoxide anion (O₂⁻) or hydrogen peroxide (H₂O₂), ·OH exhibits an extremely high reactivity that enables it to rapidly interact with almost all biological macromolecules, including lipids, proteins, and nucleic acids, without significant specificity. This non-selective reactivity makes it a primary driver of oxidative damage in organisms.
Mechanisms of Hydroxyl Free Radical-Mediated Damage: ·OH can initiate lipid peroxidation, a chain reaction that breaks down the unsaturated fatty acids in cell membranes. This process not only destroys the structural integrity of cell membranes but also generates secondary toxic products (e.g., malondialdehyde) that further exacerbate cellular damage. For proteins, ·OH oxidizes amino acid residues (such as tyrosine, cysteine, and methionine), leading to protein denaturation, aggregation, or cleavage—ultimately impairing their biological functions (e.g., enzyme activity, structural support). In terms of nucleic acids, ·OH directly attacks the sugar-phosphate backbone and bases of DNA, causing strand breaks, base modifications (e.g., 8-hydroxy-2'-deoxyguanosine formation), and even mutations, which are closely linked to the development of cancer and aging-related genetic instability.
Significance in Research and Industry: The close association between・OH and various pathological processes (including neurodegenerative diseases like Alzheimer's and Parkinson's, cardiovascular diseases, cancer, and aging) has made the assessment of hydroxyl free radical scavenging capacity a critical research focus. In the field of antioxidant research, this capacity serves as a key indicator to evaluate the efficacy of natural or synthetic antioxidants—whether they are extracted from plants, formulated into functional foods, or developed as pharmaceutical candidates. For example, in the development of anti-aging supplements, researchers rely on accurate measurements of hydroxyl free radical scavenging ability to screen potential active ingredients; in food science, this indicator helps assess the oxidative stability of food products and the effectiveness of added antioxidants. Additionally, in environmental toxicology, monitoring the hydroxyl free radical scavenging capacity of environmental samples (e.g., water, soil extracts) can provide insights into the impact of pollutants on biological oxidative balance.
Role of the Assay Kit: Traditional methods for detecting hydroxyl free radical scavenging capacity, such as high-performance liquid chromatography (HPLC) or electron spin resonance (ESR), often suffer from limitations including high instrument costs, complex operational procedures, long detection times, and poor compatibility with high-throughput sample analysis. Our Hydroxyl Free Radical Scavenging Capacity Assay Kit addresses these challenges by offering a rapid, sensitive, and user-friendly solution. It enables researchers in academia, pharmaceutical development, food science, and environmental monitoring to efficiently quantify the hydroxyl free radical scavenging ability of various samples, providing reliable data support for their studies on oxidative stress, antioxidant screening, and related disease mechanisms.
Broad Sample Compatibility: The kit is validated for use with common biological samples such as serum, plasma, and tissue homogenates, making it suitable for a wide range of research scenarios—from basic biological studies on animal models to clinical sample analysis (for research purposes only). It can also be adapted for testing other sample types (e.g., cell lysates, plant extracts, and food extracts) with simple preprocessing, eliminating the need for multiple kits for different sample sources.
Rapid Detection Workflow: With a total detection time of only 40 minutes, the kit significantly reduces the time required for experimental analysis compared to traditional methods (which often take several hours). The streamlined workflow involves just three core steps: sample addition, incubation, and absorbance measurement—minimizing operational complexity and reducing the risk of human error during long experimental processes.
High Detection Accuracy and Reproducibility: The kit exhibits low inter-assay variation (4.6%) and intra-assay variation (4.1%), ensuring consistent and reliable results across different experiments and operators. This high reproducibility is crucial for research projects that require large-scale sample testing or long-term data accumulation (e.g., longitudinal studies on antioxidant efficacy in animal models).
Optimal Detection Wavelength and Instrument Compatibility: The kit is designed for use with a microplate reader, with a detection wavelength range of 500–530 nm and an optimal wavelength of 510 nm. This wavelength range is widely supported by most standard microplate readers in laboratories, eliminating the need for specialized or expensive detection equipment and reducing the barrier to entry for use.
Stable Storage and Long Shelf Life: The kit can be stored at 2–8°C (protected from light) for up to 12 months, providing excellent stability and convenience for laboratory inventory management. There is no need for ultra-low temperature storage (e.g., -20°C or -80°C), which simplifies storage conditions and reduces the risk of component degradation during transportation or storage.
Research-Ready Design: The kit includes all necessary reagents and clear, step-by-step instructions for operation. It also provides guidance on sample size (20 μL per sample) and recommended sample preprocessing methods (if applicable), ensuring that even researchers with limited experience in oxidative stress assays can quickly master the experimental process and obtain valid results.
Cost-Effective Compared to Traditional Methods: Compared to HPLC or ESR, which require expensive instruments and consumables, our kit reduces the cost per test by more than 50%. This cost advantage is particularly beneficial for laboratories with limited budgets or those that need to process a large number of samples (e.g., high-throughput screening of antioxidant compounds), allowing them to allocate resources more efficiently to other research needs.
Superior Sensitivity for Trace Sample Detection: The kit’s optimized reaction system enables the detection of trace amounts of hydroxyl free radical scavengers in samples, even when working with small sample volumes (20 μL). This is especially valuable for studies where sample availability is limited (e.g., rare clinical samples, primary cell cultures with low yields), as it avoids the need for large sample collection and minimizes sample waste.
Strong Anti-Interference Capability: The kit’s reaction system is formulated with components that reduce non-specific interference from metal ions and other impurities commonly present in biological or food samples. This ensures that the measured absorbance values accurately reflect the hydroxyl free radical scavenging capacity of the target analytes, rather than being affected by background contaminants—improving the reliability of experimental data.
Flexible Adaptability to Experimental Scales: Available in two sizes (96 T for 92 samples and 48 T for 44 samples), the kit can be tailored to different experimental needs. The 96 T size is ideal for high-throughput studies (e.g., screening libraries of antioxidant compounds), while the 48 T size is more suitable for small-scale experiments (e.g., preliminary validation of a few candidate samples), helping laboratories avoid unnecessary reagent waste.
For research use only, not for clinical use.
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