| Product Name | Human Leukemia Inhibitory Factor (LIF) ELISA Kit |
| Catalog No. | BDDK-HMM-0026 |
| Description | Leukemia inhibitory factor (LIF) is an interleukin 6 family cytokine that affects cell growth by inhibiting differentiation. When LIF levels decrease, cells differentiate. Activated T cells, monocytes, astrocytes, osteoblasts, keratinocytes, mast cells, and fibroblasts can express LIF.LIF is named for its ability to induce terminal differentiation of myeloid leukemia cells and to prevent the continued growth of leukemia cells.Other properties of LIF include growth promotion, promotion of the differentiation of different types of target cells, effects on bone metabolism, malignant stigmata, neural development embryonic development and inflammation. |
| Disease Type | Leukaemia |
| Applications | This test kit is for the quantitative detection of Leukemia Inhibitory Factor (LIF) by enzyme-linked immunosorbent assay. |
| Sample Type | Serum, plasma, cell culture supernatants and other biological samples |
| Detection Methods | Sandwich ELISA |
| Sensitivity | 0.64 pg/mL |
| Detection Range | 15.63-1000 pg/mL |
| Accuracy | Intraplate coefficient of variation: 3.6%-3.8%; Interplate coefficient of variation: 2.0%-4.8% |
| Storage | Reagent kit is not opened, stored at 4°C. Disassembled, store at -20°C for standards, 4°C for others. |
| Detection Principle | This kit uses double antibody sandwich enzyme-linked immunosorbent assay technology. The specific anti-human LIF antibody is pre-coated on a high affinity enzyme labeled plate. Standards and samples to be tested are added to the wells of the enzyme plate, and after incubation, the LIF present in the samples binds to the solid phase antibody. After washing to remove unbound material, a biotinylated detection antibody is added and incubated. The unbound biotinylated antibody is washed and horseradish peroxidase-labeled streptavidin (Streptavidin-HRP) is added. After washing, the color development substrate TMB is added and the color is developed away from light. The depth of the color reaction is proportional to the concentration of LIF in the sample. The reaction was terminated by adding termination solution and the absorbance value was measured at 450 nm (reference wavelength 570 - 630 nm). |
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