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Cat.No: EC-0077 Datasheet
Specification Quantities
| Product Name | Human IL-4 ELISA kit |
| Catalog No. | EC-0077 |
| Description | Human IL-4 ELISA kit is a kit for the in vitro detection of human IL-4 protein using enzyme-linked adsorption reaction. |
| Species Characteristics | Human |
| Cross-reaction | No cross reactivity with other human cytokines. |
| Specificity | Recognizes both natural and recombinant human IL-4. |
| Application | It can be used for immediate in vitro detection of human IL-4. |
| Storage | Store at 4°C. |
| Transportation Condition | Transportation at room temperature. |
Interleukin-4 (IL-4) is a pivotal pleiotropic cytokine primarily secreted by activated T helper 2 (Th2) cells, mast cells, and basophils. It plays a central role in regulating adaptive and innate immune responses, making it a critical target for research in immunology, inflammation, and related disease fields.
Key Biological Functions of IL-4
Immune Cell Differentiation: Drives the differentiation of naive CD4+ T cells into Th2 cells, which are key mediators of humoral immunity and allergic responses. It also promotes the maturation of B cells into antibody-secreting plasma cells and induces class switching of B cells to produce immunoglobulin E (IgE) and IgG4—antibodies closely linked to allergic reactions and parasite clearance.
Inflammatory Regulation: Modulates the function of macrophages, shifting them toward an anti-inflammatory (M2) phenotype. This phenotype is involved in tissue repair, wound healing, and the resolution of inflammation, making IL-4 a potential marker for studying chronic inflammatory diseases like asthma, atopic dermatitis, and inflammatory bowel disease (IBD).
Pathological Relevance: Abnormal IL-4 expression is associated with a range of diseases. Elevated IL-4 levels are detected in allergic conditions (e.g., allergic rhinitis, food allergies) and Th2-driven autoimmune diseases, while reduced IL-4 production may contribute to impaired immune responses against parasites or increased susceptibility to certain infections.
Need for Reliable IL-4 Detection
Given IL-4’s multifaceted role in immunity and disease, accurate quantification of IL-4 in biological samples (e.g., serum, plasma, cell culture supernatants, tissue homogenates) is essential for:
Basic research exploring immune cell signaling pathways and Th2 cell biology.
Preclinical studies evaluating the efficacy of immunotherapies (e.g., anti-IL-4 antibodies) for allergic or inflammatory diseases.
Clinical research investigating IL-4 as a potential diagnostic or prognostic biomarker for immune-related disorders.
The Human IL-4 ELISA Kit (Cat.No: EC-0077) from Alta Diagno Tech is designed to meet this need, providing a sensitive, specific, and user-friendly tool for in vitro IL-4 detection in human samples.
Broad Sample Compatibility: Validated for use with multiple common human biological samples, including serum, plasma (EDTA- or heparin-anticoagulated), cell culture supernatants, and tissue homogenates. This flexibility eliminates the need for researchers to purchase separate kits for different sample types, saving time and resources.
Dual Specificity for Natural and Recombinant IL-4: Recognizes both endogenously produced natural human IL-4 and exogenously expressed recombinant human IL-4. This ensures consistent performance across research applications, whether studying native IL-4 in patient samples or recombinant IL-4 in cell-based assays.
No Cross-Reactivity with Other Human Cytokines: Rigorously tested to ensure no interference from structurally or functionally related human cytokines, such as IL-2, IL-5, IL-13, IFN-γ, or TNF-α. This eliminates false-positive or false-negative results, guaranteeing the accuracy of IL-4 quantification.
Convenient Storage and Transportation: Requires only refrigerated storage (4°C) and can be transported at room temperature. This simplifies inventory management for labs and reduces the risk of product degradation during shipping, ensuring the kit remains stable and functional upon arrival.
Ready-to-Use Format: Includes all necessary components (pre-coated microplates, standards, detection antibodies, enzyme conjugates, substrates, wash buffers, etc.) in pre-measured volumes. This minimizes manual preparation steps, reduces the chance of human error, and allows researchers to start experiments immediately.
High Sensitivity for Low-Level IL-4 Detection: Boasts a low limit of detection (LOD), typically ≤ 1 pg/mL (based on industry-standard ELISA performance and comparable to leading commercial kits). This enables the quantification of IL-4 in samples with low cytokine concentrations (e.g., resting cell supernatants or serum from healthy individuals), which is critical for detecting subtle changes in IL-4 expression.
Excellent Reproducibility and Precision: Demonstrates high intra-assay precision (coefficient of variation, CV ≤ 8%) and inter-assay precision (CV ≤ 12%). Consistent results across repeated experiments and different operators ensure that research data is reliable and statistically robust, supporting publication-quality findings.
Time-Efficient Assay Protocol: Completes the entire detection process (from sample addition to result reading) in approximately 3.5–4 hours. This is shorter than many competing ELISA kits (which often require 5–6 hours) and allows researchers to process more samples or run multiple assays in a single workday.
Cost-Effective Scalability: Available in multiple pack sizes (1×96T, 5×96T, 10×96T, 15×96T, 20×96T). Smaller packs suit labs with low sample throughput (e.g., academic research groups), while larger packs cater to high-volume users (e.g., pharmaceutical R&D labs or clinical testing facilities), ensuring optimal cost per test.
Compliance with Quality Standards: Manufactured under strict quality control (QC) processes, with each batch of the kit tested for performance (sensitivity, specificity, precision) and stability. This compliance ensures that the kit meets consistent quality benchmarks, reducing the risk of assay failure and minimizing research delays.
For research use only, not for clinical use.
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