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| Product Name | Human HCG-IgG (Human Chorionic Gonadotropin-Immunoglobulin G) ELISA Kit |
| Catalog No. | HTK-YJL-0005 |
| Synonyms | HCG IgG ELISA Kit, Human Chorionic Gonadotropin-Immunoglobulin G ELISA Kit |
| Species | Human |
| Sample Type | Serum, plasma, cell culture supernatant, cell or tissue lysate, other liquid samples |
| Detection Method | Indirect ELISA |
| Detection Wavelength | OD450 |
| Reaction Duration | 3 hours |
| Range | Qualitative |
| Sensitivity | Qualitative |
| Storage | 2-8°C (Sealed). Don't cryopreserve. |
| Specificity | Specifically binds with HCG-IgG, no obvious cross reaction with other analogues. |
| Intended Use | For research use only. |
Human Chorionic Gonadotropin (HCG) is a critical glycoprotein hormone primarily produced by the trophoblast cells of the placenta during pregnancy. It plays a pivotal role in maintaining the corpus luteum, which in turn secretes progesterone to support the early stages of pregnancy. Beyond normal pregnancy, HCG is also associated with specific pathological conditions, such as hydatidiform mole, choriocarcinoma, and certain germ cell tumors—making it a key biomarker for both physiological and pathological research.
Immunoglobulin G (IgG), the most abundant antibody in human serum, is involved in recognizing and binding to specific antigens, including HCG. The formation of HCG-IgG complexes has gained significant attention in reproductive biology, oncology, and immunology research, as it may reflect immune responses to HCG-related processes or abnormal HCG secretion in disease states.
The Human HCG-IgG ELISA Kit is developed to address the growing demand for reliable tools to detect HCG-IgG in biological samples. In research settings, this kit enables scientists to:
Explore the correlation between HCG-IgG levels and reproductive disorders, such as recurrent pregnancy loss or ectopic pregnancy, by analyzing serum or plasma samples.
Investigate the role of HCG-IgG in tumor immunology, particularly in studies focusing on HCG-secreting tumors (e.g., testicular choriocarcinoma, ovarian germ cell carcinomas), to understand immune interactions with tumor-derived HCG.
Evaluate HCG-IgG presence in cell culture supernatants or tissue lysates, supporting in vitro studies on cell-specific HCG secretion and subsequent immune responses.
Contribute to the development of novel research models for HCG-related immunological mechanisms, as IgG-based immune responses are often central to understanding antigen-antibody interactions in disease progression.
Broad Sample Compatibility: This kit is designed to process multiple types of biological samples commonly used in research, including serum, plasma, cell culture supernatant, and cell or tissue lysate. This flexibility eliminates the need for researchers to invest in separate kits for different sample types, streamlining experimental workflows.
Specific Target Binding: The kit exhibits high specificity for HCG-IgG, with no obvious cross-reactivity with other HCG analogs (e.g., free HCG alpha/beta subunits) or unrelated immunoglobulins. This ensures that the detected signal accurately reflects HCG-IgG levels, minimizing false-positive or false-negative results in research data.
Time-Efficient Assay Protocol: With a total reaction duration of 3 hours, the kit enables researchers to obtain results within a single working session. This short turnaround time is particularly beneficial for high-throughput research projects or studies requiring rapid sample analysis.
Stable Storage Conditions: The kit can be stored at 2-8°C in a sealed state, without the need for cryopreservation. This simplifies inventory management for laboratories, as it avoids the risks of reagent degradation associated with repeated freeze-thaw cycles and reduces the need for specialized storage equipment.
Indirect ELISA Detection Method: Leveraging the indirect ELISA technique—an established, reliable method in immunoassay research—this kit ensures consistent and reproducible detection of HCG-IgG. The method is widely recognized in the scientific community for its sensitivity to antibody-antigen complexes, making it suitable for qualitative analysis of HCG-IgG in research samples.
Research Reliability: By minimizing cross-reactivity with non-target molecules, the kit provides trustworthy qualitative data on HCG-IgG presence. This reliability is critical for research studies, as it ensures that experimental conclusions are based on accurate detection of the target analyte, reducing the risk of misinterpretation of results.
User-Friendly Workflow: The kit’s protocol is designed to be straightforward, requiring no specialized technical expertise beyond basic laboratory skills. This accessibility allows researchers across different experience levels to use the kit effectively, reducing the learning curve and potential operational errors.
Cost-Effective Sample Processing: The ability to handle multiple sample types (serum, plasma, cell lysates, etc.) means laboratories do not need to purchase separate kits for each sample matrix. This reduces overall research costs while maintaining consistent assay performance across different experimental setups.
Consistent Performance: The kit’s components are manufactured to strict quality standards, ensuring batch-to-batch consistency. This is essential for long-term research projects or studies requiring repeated sample analysis, as it guarantees that results from different experiments (or different kit batches) are comparable.
Support for Diverse Research Areas: Whether used in reproductive biology, oncology, or immunology research, the kit adapts to various research objectives—from studying pregnancy-related immune responses to investigating tumor-associated HCG-IgG. This versatility makes it a valuable asset for laboratories with multiple research focuses.
For research use only, not for clinical use.
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