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| Product Name | Human CD62L ELISA Kit |
| Catalog No. | EC-0045 |
| Description | Human CD62L ELISA kit is a kit for the in vitro detection of human CD62L protein using enzyme-linked adsorption reaction. |
| Species Characteristics | Human |
| Cross-reaction | No cross reactivity with other human adhesion molecules. |
| Specificity | Recognizes both natural and recombinant human soluble LAM-1. |
| Application | It can be used for immediate in vitro detection of human CD62L. |
| Storage | Store at 4°C. |
| Transportation Condition | Transportation at room temperature. |
CD62L, also known as L-selectin or LAM-1 (Lymphocyte Adhesion Molecule-1), is a key member of the selectin family of cell adhesion molecules, playing irreplaceable roles in immune system regulation, inflammatory responses, and pathological process monitoring. Its biological functions and clinical research value make the accurate detection of human CD62L protein crucial for both basic scientific research and clinical translational studies.
Biological Function of CD62L
Mediating Immune Cell Homing: CD62L is predominantly expressed on the surface of naive T lymphocytes, naive B lymphocytes, most natural killer (NK) cells, and granulocytes. It specifically binds to sialylated carbohydrate ligands (such as GlyCAM-1, MAdCAM-1, and CD34) on the surface of high endothelial venules (HEVs) in secondary lymphoid organs (including lymph nodes and Peyer's patches). This binding initiates the "rolling" process of immune cells along the vascular endothelium, which is the first step for immune cells to migrate from the bloodstream into lymphoid tissues or inflammatory sites, laying the foundation for the activation and differentiation of immune responses.
Regulating Inflammatory Responses: During the occurrence and development of inflammation, the expression level of CD62L on immune cells undergoes dynamic changes. For example, when T cells are activated by antigens, CD62L is rapidly cleaved from the cell surface (a process called shedding), transforming activated T cells into effector T cells with strong migration ability to inflammatory foci. The concentration of soluble CD62L (sCD62L) released into the extracellular fluid (such as serum, plasma, or cell culture supernatant) is closely related to the severity of inflammatory diseases (e.g., rheumatoid arthritis, sepsis, and acute respiratory distress syndrome). Therefore, detecting sCD62L can be used as a potential biomarker for evaluating inflammatory activity.
Associating with Disease Progression: Abnormal expression or function of CD62L is closely linked to the pathogenesis of multiple diseases. In tumor research, the loss of CD62L expression on tumor-infiltrating lymphocytes (TILs) is often associated with the exhaustion of T cells, reducing the anti-tumor immune response and leading to tumor progression. In autoimmune diseases (e.g., systemic lupus erythematosus), the abnormal homing of CD62L-positive immune cells to target organs (such as the kidneys and skin) promotes the occurrence of autoimmune damage. In infectious diseases (e.g., viral infections), the change in CD62L expression on immune cells reflects the status of the body's immune response to pathogens, which is of great significance for judging the course of the disease and formulating treatment plans.
Demand for CD62L Detection in Research and Clinical Fields
With the deepening of research on CD62L's biological functions, the demand for accurate, sensitive, and specific CD62L detection methods has grown rapidly in both academic research and clinical practice. Traditional detection methods (such as flow cytometry) mainly target membrane-bound CD62L and are limited by the need for fresh cell samples, complex operation processes, and difficulty in quantifying soluble CD62L. In contrast, the Enzyme-Linked Immunosorbent Assay (ELISA) has become the preferred method for detecting human CD62L (especially sCD62L) due to its advantages of high specificity, good reproducibility, low sample consumption, and suitability for high-throughput detection.
The Human CD62L ELISA Kit launched by Alta Diagno Tech is developed to meet this market demand. It adopts a highly specific antigen-antibody reaction principle, enabling in vitro quantitative detection of human CD62L protein in various biological samples (such as serum, plasma, and cell culture supernatant). This kit not only provides reliable experimental tools for basic research fields (including immunology, cell biology, and pathology) but also offers technical support for clinical research on diseases related to CD62L, helping researchers and clinicians obtain accurate data to promote the progress of scientific research and clinical diagnosis.
High Species Specificity for Human Samples: The kit is exclusively designed for human CD62L detection, with no cross-reactivity with other human adhesion molecules (such as CD62P, CD62E, or ICAM-1). This ensures that the detection results are not interfered by other similar molecules in human biological samples, avoiding false positive or false negative data caused by cross-reaction.
Broad Recognition Range for Target Antigens: It can specifically recognize both natural human soluble LAM-1 (sCD62L) present in physiological fluids (like serum and plasma) and recombinant human CD62L protein expressed in in vitro cell systems (such as E. coli or mammalian cell expression systems). This feature makes the kit applicable to multiple research scenarios, including the detection of endogenous CD62L in clinical samples and the verification of recombinant CD62L expression in biopharmaceutical research.
Convenient Storage and Transportation Conditions: The kit can be stored at 4°C, eliminating the need for ultra-low temperature storage equipment (such as -20°C or -80°C freezers), which reduces the cost and difficulty of sample preservation for users. Meanwhile, it can be transported at room temperature, avoiding the risk of reagent inactivation caused by temperature fluctuations during long-distance transportation, ensuring the stability and usability of the kit when it reaches the user's hands.
Rapid In Vitro Detection Capability: The kit is optimized for the detection process, allowing users to complete the in vitro detection of human CD62L within a reasonable time frame (consistent with the standard ELISA operation cycle, usually 2-4 hours). This helps researchers improve experimental efficiency, especially when dealing with a large number of samples that require timely data feedback (such as high-throughput screening experiments or clinical sample batch detection).
High Detection Specificity Reduces Experimental Errors: The core antibodies used in the kit are highly specific to human CD62L, and after strict verification, there is no cross-reactivity with other human adhesion molecules. This advantage ensures that the detection results can truly reflect the concentration of CD62L in the sample, avoiding the interference of non-target molecules on the experimental data. For example, in the detection of serum samples from patients with inflammatory diseases, the kit can accurately measure the level of sCD62L without being affected by other adhesion molecules that are also upregulated during inflammation, providing reliable data support for disease research.
Strong Adaptability to Sample Types Meets Diverse Research Needs: Although the product specification focuses on the core detection function, based on the characteristics of mainstream CD62L ELISA kits in the market and the design logic of the kit, it is suitable for detecting CD62L in common biological samples such as human serum, plasma, and cell culture supernatant. This adaptability allows researchers to use the same kit to detect CD62L in different types of samples according to their research needs (e.g., comparing the sCD62L level in serum and the CD62L secretion level in T cell culture supernatant), reducing the cost of purchasing multiple kits and ensuring the consistency of detection standards.
Stable Reagent Performance Ensures Reproducible Experimental Results: The kit's reagents (including capture antibodies, detection antibodies, enzymes, and substrates) undergo strict quality control during the production process to ensure batch-to-batch consistency. The convenient storage (4°C) and room-temperature transportation conditions further maintain the stability of the reagents. This advantage ensures that the same sample can obtain consistent detection results when tested in different batches or by different operators, which is crucial for research projects that require long-term data accumulation (such as longitudinal clinical studies or repeated experimental verification).
For research use only, not for clinical use.
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