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| Product Name | Free Cholesterol (FC) Colorimetric Assay Kit |
| Catalog No. | CMAK-YJL-0112 |
| Size | 96 T (92 samples), 48 T (44 samples) |
| Sample Type | Serum (plasma); tissues |
| Detection Instrument | Microplate reader (495-525 nm, optimal detection wavelength: 510 nm) |
| Research Field | Glucose and lipid metabolism |
| Sensitivity | 0.07 mmol/L |
| Detection Range | 0.07-24 mmol/L |
| Accuracy | Inter-assay variation: 5.2% | Intra-assay variation: 1.9% |
| Sample Size | 5 μL |
| Storage | The product can be stored at 2-8°C, protected from light for 6 months. |
| Shelf Life | 6 months |
| Index Abbreviation | FC |
| Detection Time | 20 min |
| Intended Use | For research use only. |
Free cholesterol (FC) is a critical lipid molecule with indispensable roles in biological systems, making its accurate quantification essential for advancing research in glucose and lipid metabolism, as well as related disease mechanisms.
Biological Significance of Free Cholesterol: FC serves as a fundamental structural component of cell membranes, where it regulates membrane fluidity, permeability, and the function of membrane-embedded proteins—key factors in maintaining cellular integrity and normal physiological processes. Beyond membrane structure, FC acts as a vital precursor for the biosynthesis of essential biomolecules, including adrenal cortex hormones (which regulate metabolism, immune response, and stress adaptation), sex hormones (critical for reproductive development and function), bile acids (essential for fat digestion and absorption in the intestine), and vitamin D (crucial for calcium homeostasis and bone health). Additionally, FC levels in biological samples (such as serum, plasma, and tissues) are closely linked to the formation, absorption, and catabolism of cholesterol in the liver and intestines, as well as the metabolism of lipoproteins in the bloodstream. Abnormal FC concentrations are often associated with lipid metabolism disorders, making it a key biomarker for studying conditions related to lipid imbalance.
Evolution of FC Detection Methods: Early approaches for measuring FC relied on chemical colorimetric methods, which often suffered from limitations such as low specificity (susceptible to interference from other lipids or biological molecules), narrow detection ranges, and relatively complex operational procedures. These drawbacks made it challenging to obtain precise and reproducible results, hindering progress in lipid metabolism research. With advancements in enzymatic technology, modern FC detection has shifted to enzymatic colorimetric assays. Our Free Cholesterol (FC) Colorimetric Assay Kit leverages this advanced technology to address the shortcomings of traditional methods, enabling more accurate, efficient, and reliable FC quantification.
Research Application Context: In the field of glucose and lipid metabolism research, understanding FC dynamics is critical for unraveling the intricate mechanisms underlying metabolic pathways and their dysregulation in diseases. Researchers frequently need to measure FC levels in various biological samples (e.g., serum from human or animal models, tissue homogenates from organs like the liver or kidney) to investigate how dietary factors, genetic variations, or drug treatments affect lipid metabolism. Our assay kit is specifically designed to meet these research needs, providing a robust tool that supports high-quality data collection for studies on lipid biology, metabolic disorders, and related therapeutic developments.
Broad Sample Compatibility: The kit is optimized for use with multiple common biological sample types, including serum, plasma, and tissue samples. This flexibility eliminates the need for researchers to purchase separate kits for different sample sources, simplifying experimental workflows and reducing overall research costs.
High Sensitivity and Wide Detection Range: It offers a sensitivity of 0.07 mmol/L, enabling the detection of low FC concentrations that may be critical for studying early-stage metabolic changes or subtle differences between experimental groups. Additionally, the kit provides a wide detection range of 0.07–24 mmol/L, which covers the typical FC concentration ranges found in most biological samples, eliminating the need for excessive sample dilution or concentration steps that can introduce errors.
Excellent Accuracy and Precision: The kit ensures reliable results with low inter-assay variation (5.2%) and intra-assay variation (1.9%). Low inter-assay variation means consistent performance across different assay runs, while low intra-assay variation ensures uniformity of results within a single experiment—both are essential for generating reproducible data that meets rigorous scientific standards.
Rapid Assay Time: With a total detection time of just 20 minutes, the kit significantly reduces the time required for FC quantification compared to slower traditional methods. This allows researchers to process more samples in a shorter period, accelerating experimental progress and increasing overall research efficiency.
Convenient Storage Conditions: The product can be stored at 2–8°C (a standard refrigerator temperature) and protected from light for 6 months. This eliminates the need for specialized storage equipment (such as -20°C or -80°C freezers) and simplifies inventory management, as researchers can easily stock the kit without worrying about complex storage requirements.
Minimal Sample Requirement: Only 5 μL of sample is needed per assay. This is particularly beneficial for researchers working with limited sample volumes (e.g., samples from small animal models, rare specimens, or hard-to-obtain tissue samples), as it allows for FC measurement without depleting valuable sample resources.
User-Friendly Operation: The kit follows a straightforward protocol that does not require advanced technical skills or specialized training. Clear, step-by-step instructions guide researchers through sample preparation, reagent addition, and detection, minimizing the risk of operational errors and ensuring consistent results even for less experienced laboratory personnel.
Cost-Effective Design: Available in two sizes (96 T for 92 samples and 48 T for 44 samples), the kit allows researchers to choose the appropriate volume based on their experimental needs. This prevents unnecessary waste of reagents (for small-scale experiments) or frequent reordering (for large-scale studies), optimizing cost efficiency and aligning with budget constraints.
Reliable Performance with Validated Results: The assay’s principles and performance metrics (sensitivity, accuracy, precision) are consistent with industry standards for lipid quantification assays. This validation ensures that the results obtained using the kit are trustworthy and comparable to data from other validated methods, enhancing the credibility of research findings and supporting their publication in peer-reviewed journals.
Compatibility with Standard Laboratory Equipment: It is designed to work with a microplate reader (detection wavelength: 495–525 nm, optimal at 510 nm)—a common piece of equipment in most research laboratories. Researchers do not need to invest in expensive, specialized instruments to use the kit, reducing barriers to adoption and making FC quantification accessible to a wider range of research teams.
Research-Focused Design: The kit is explicitly developed for research use, with features tailored to the unique needs of lipid metabolism researchers. For example, its ability to handle tissue samples (in addition to serum and plasma) supports studies on organ-specific lipid metabolism, while its rapid assay time and minimal sample requirement align with the high-throughput, resource-efficient demands of modern research.
For research use only, not for clinical use.
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