| Product Name | Fluorescence In Situ Hybridization Kit for RNA |
| Catalog No. | IR-HMM-0087 |
| Description | The RNA Fluorescence In Situ Hybridization Kit is a highly sensitive detection kit. It utilizes the principle of base complementarity to hybridize fluorescently labeled oligonucleotide probes (DNA or RNA) with target RNA in cells or tissues. After hybridization, fluorescently labeled nucleic acid probe-target RNA hybridization complexes are formed, which can be observed and analyzed under a fluorescence microscope. |
| Applications | This product is used to observe and determine the cellular localization of target RNA in cells or tissues. |
| Detection Principle | It utilizes the principle of base complementarity to hybridize fluorescently labeled oligonucleotide probes (DNA or RNA) with target RNA in cells or tissues. After hybridization, fluorescently labeled nucleic acid probe-target RNA hybridization complexes are formed, which can be observed and analyzed under a fluorescence microscope. |
| Component | Proteinase K (20 mg/mL) Acetylation Solution A Acetylation Solution B Hybridization Solution Yeast RNA (100X) DAPI (5 mg/mL) Wash Buffer (20X) |
| Materials Required But Not Provided | Ultrapure Water (DNase/RNase-Free, Sterile) or DEPC Water 4% Paraformaldehyde Fixative Solution PBS, pH7.3 (DNase, RNase & Protease free, Sterile) Hydrochloric Acid Deionized Formamide Anti-Fluorescence Quenching Mounting Solution Poly-L-Lysine (prepare as needed) Cover Glasses (prepare as needed) Reagents related to frozen or paraffin sections (prepare as needed) Target RNA Fluorescent Probes (must be purchased or synthesized independently) |
| Storage | -20°C |
For in vitro diagnostic use.
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