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| Product Name | DHT (Dihydrotestosterone) ELISA Kit |
| Catalog No. | HTK-YJL-0011 |
| Synonyms | DHT ELISA Kit, Dihydrotestosterone ELISA Kit |
| Species | Universal |
| Sample Type | Serum, plasma, cell culture supernatant, cell or tissue lysate, other liquid samples |
| Detection Method | Competitive ELISA, Coated with Antigen |
| Detection Wavelength | OD450 |
| Reaction Duration | 2 hours |
| Range | 39.063-2500 pg/ml |
| Sensitivity | 23.438 pg/ml |
| Storage | 2-8°C (Sealed). Don't cryopreserve. |
| Specificity | Specifically binds with DHT, no obvious cross reaction with other analogues. |
| Intended Use | For research use only. |
Dihydrotestosterone (DHT), a key endogenous androgenic steroid hormone, plays a pivotal role in numerous physiological and pathological processes across species, making its accurate quantification critical for both basic research and translational studies.
Biosynthesis and Physiological Significance: DHT is synthesized from testosterone through the catalytic action of 5α-reductase, an enzyme abundantly expressed in target tissues such as the prostate, seminal vesicles, epididymis, skin, hair follicles, liver, and brain. Unlike testosterone, DHT exhibits significantly higher affinity (up to 5 times stronger) for the androgen receptor (AR), enabling it to exert more potent androgenic effects. In males, it is essential for the development of external genitalia during embryogenesis, the growth of facial and body hair during puberty, and the maintenance of prostate function in adulthood. In females, DHT is present at lower concentrations but contributes to follicular development and bone density regulation.
Pathological Implications in Research: Abnormal DHT levels are closely linked to a range of hormone-related disorders, driving the demand for reliable detection tools. For instance:
Androgenetic alopecia (male/female pattern baldness): Elevated DHT in hair follicles induces miniaturization of follicles, leading to progressive hair thinning and loss.
Prostate disorders: Benign Prostatic Hyperplasia (BPH) and prostate cancer are associated with excessive DHT stimulation of prostate epithelial cell proliferation.
Hormonal imbalances: Polycystic Ovary Syndrome (PCOS) in females often involves increased DHT production, contributing to symptoms like hirsutism and acne.
These pathological connections make DHT a key biomarker for studying the mechanisms of such diseases and evaluating potential therapeutic interventions (e.g., 5α-reductase inhibitors).
Need for Sensitive and Specific Detection: Traditional methods for DHT quantification, such as gas chromatography-mass spectrometry (GC-MS) or liquid chromatography-tandem mass spectrometry (LC-MS/MS), offer high accuracy but require expensive equipment, specialized technical expertise, and long assay times—limiting their accessibility for routine research. ELISA kits address these limitations by providing a cost-effective, user-friendly, and high-throughput alternative, enabling laboratories of all sizes to perform quantitative DHT analysis with minimal equipment investment. Our DHT ELISA Kit is designed to meet this demand, combining specificity, sensitivity, and efficiency to support diverse research applications.
Universal Species Compatibility: Designed to detect DHT across a wide range of species (including human, mouse, rat, and other mammals). This flexibility eliminates the need for researchers to source species-specific kits, simplifying experimental design and reducing costs when working with multiple animal models or cross-species studies.
Broad Sample Versatility: Validated for use with multiple biological sample types, including serum, plasma, cell culture supernatant, cell lysate, and tissue homogenate. This versatility allows researchers to analyze DHT in different biological matrices, supporting studies on systemic hormone levels (serum/plasma) and local tissue-specific DHT production (cells/tissues).
Rapid Assay Workflow: Completes the entire detection process in just 2 hours, significantly reducing experimental time compared to traditional MS-based methods (which can take hours to days) or slower ELISA kits (3-4 hours). This efficiency enables high-throughput screening of large sample sets, accelerating research progress.
Reliable Sensitivity and Dynamic Range: Offers a sensitivity of 23.438 pg/ml and a dynamic detection range of 39.063–2500 pg/ml. The low sensitivity ensures accurate quantification of low DHT concentrations (e.g., in female serum or small-volume cell supernatants), while the broad range covers physiological and pathological DHT levels, avoiding the need for multiple dilutions of high-concentration samples (e.g., prostate tissue lysates).
Exceptional Specificity: Specifically binds to DHT with no obvious cross-reactivity with structurally similar steroids (such as testosterone, 5β-DHT, androstenedione, or estrogens). This high specificity prevents false-positive or false-negative results, ensuring the reliability of experimental data—critical for studies where DHT levels are compared to other steroid hormones.
Stable Storage Conditions: Can be stored at 2–8°C in a sealed container (no cryopreservation required). This simplifies inventory management, as the kit does not need to be stored in a -20°C freezer, reducing the risk of component degradation due to repeated freeze-thaw cycles.
Cost-Effective High-Throughput Analysis: Compared to MS-based methods (which cost thousands of dollars per instrument and require costly reagents), our ELISA kit provides an affordable solution for routine DHT quantification. Each kit (48T/96T) supports multiple assays, lowering the per-sample testing cost and making it ideal for laboratories with budget constraints or large sample cohorts.
User-Friendly Protocol: Requires no specialized technical skills or advanced equipment (only a standard microplate reader with a 450 nm wavelength). The protocol is straightforward, with clear step-by-step instructions, reducing the learning curve for new users and minimizing human error during operations (e.g., pipetting, washing).
Consistent Reproducibility: Ensures low intra-assay and inter-assay variation (consistent with industry standards for ELISA kits). This reproducibility guarantees that results can be replicated across different experiments, operators, or time points—essential for publishing reliable research data and validating experimental findings.
Time-Saving Sample Preparation: Does not require complex sample pre-treatment (e.g., extraction or derivatization) that is necessary for MS-based methods. Samples can be used directly after simple processing (e.g., centrifugation of serum/plasma, lysis of cells), saving valuable time and reducing the risk of sample loss during pre-treatment.
Research-Focused Design: Optimized for research applications, with performance characteristics tailored to the needs of endocrinology, oncology, dermatology, and reproductive biology research. Whether studying DHT’s role in prostate cancer progression, hair follicle biology, or hormonal disorders, the kit delivers data that meets the rigorous standards of academic and industrial research.
For research use only, not for clinical use.
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